Document Detail


An alternative method of chronic cerebrospinal fluid collection via the cisterna magna in conscious rhesus monkeys.
MedLine Citation:
PMID:  12906404     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Models of chronic cerebrospinal fluid (CSF) collection previously have been established for nonhuman primates and canines; many of these methods implement stainless-steel cannulas into the lateral or 4th ventricles or catheters into the cerebral or spinal subarachnoid space. These models have proved successful and reliable but unfortunately require invasive techniques to pass through the skull or require a laminectomy to enter the spinal subarachnoid space, involve the use of expensive and highly specialized stereotaxic equipment for the precise placement of the implants, and may require exteriorized hardware which is cumbersome to maintain and unaesthetic. The model we developed for the rhesus monkey allows for direct access to CSF outflow from the cisterna magna by using a 3.5-French fenestrated silicone catheter which was placed 1.0 cm into the cisterna. The catheter was attached to a titanium port placed subcutaneously between the scapulae to permit easy access for sampling CSF in a conscious, chaired rhesus monkey. We currently have instrumented animals from which we have consistently collected CSF for over 18 months. This novel, economical, less-invasive method permits chronic, reliable collection of CSF in conscious rhesus monkeys and has the additional advantages that the model is easier to maintain and more aesthetic.
Authors:
David B Gilberto; Angela H Zeoli; Peter J Szczerba; John R Gehret; Marie A Holahan; Gary R Sitko; Colena A Johnson; Jacquelynn J Cook; Sherri L Motzel
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Publication Detail:
Type:  Evaluation Studies; Journal Article    
Journal Detail:
Title:  Contemporary topics in laboratory animal science / American Association for Laboratory Animal Science     Volume:  42     ISSN:  1060-0558     ISO Abbreviation:  Contemp Top Lab Anim Sci     Publication Date:  2003 Jul 
Date Detail:
Created Date:  2003-08-08     Completed Date:  2003-08-26     Revised Date:  2003-11-14    
Medline Journal Info:
Nlm Unique ID:  9204153     Medline TA:  Contemp Top Lab Anim Sci     Country:  United States    
Other Details:
Languages:  eng     Pagination:  53-9     Citation Subset:  IM    
Affiliation:
Laboratory Animal Resources (LAR), Merck Research Laboratories, P.O. Box 4, Sumneytown Pike, West Point, PA 19486, USA.
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MeSH Terms
Descriptor/Qualifier:
Animals
Catheterization / methods,  veterinary*
Cerebrospinal Fluid*
Cisterna Magna*
Female
Laboratory Animal Science / methods*
Macaca mulatta*
Male
Specimen Handling / methods,  veterinary*

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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