Document Detail


An alternative, less invasive blood sample collection technique for serologic studies utilizing triatomine bugs (Heteroptera; Insecta).
MedLine Citation:
PMID:  16870877     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The collection of blood samples for serological studies is often stressful for the focus animal. Recently, the use of bloodsucking bugs, such as Dipetalogaster maximus or Triatoma infestans (Reduviidae; Triatominae; Heteroptera), has been suggested as a new and less invasive method for blood collection. To evaluate this technique, we collected paired blood samples from 20 domestic rabbits (Oryctolagus cuniculus) during a study of rabbit hemorrhagic disease virus (RHDV). For each rabbit, blood samples were collected by the conventional method (needle and syringe from the vena auricularis) and through feeding by D. maximus. Samples were tested for RHDV antibodies using standard test kits at three different dilutions. Antibody titers were identical for 56 paired samples and differed in only four cases. The simple matching indices were 1 for the 1:10 dilution and 0.9 for the 1:100 and 1:1000 dilutions. The major advantages of the new technique are 1) the possibility to obtain blood from animals where veins are inaccessible and 2) the fact that anesthesia of focus animals may not be necessary.
Authors:
Christian C Voigt; Ulrike Peschel; Gudrun Wibbelt; Kai Frölich
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Journal of wildlife diseases     Volume:  42     ISSN:  0090-3558     ISO Abbreviation:  J. Wildl. Dis.     Publication Date:  2006 Apr 
Date Detail:
Created Date:  2006-07-27     Completed Date:  2006-09-19     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0244160     Medline TA:  J Wildl Dis     Country:  United States    
Other Details:
Languages:  eng     Pagination:  466-9     Citation Subset:  IM    
Affiliation:
Institute for Zoo and Wildlife Research, Alfred-Kowalke-Str. 17, 10315 Berlin, Germany. voigt@izw-berlin.de
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MeSH Terms
Descriptor/Qualifier:
Animals
Animals, Wild / blood
Antibodies, Viral / blood
Blood Specimen Collection / methods,  veterinary*
Female
Hemorrhagic Disease Virus, Rabbit / immunology,  isolation & purification
Heteroptera / metabolism*
Male
Rabbits
Sampling Studies*
Triatominae / metabolism*
Chemical
Reg. No./Substance:
0/Antibodies, Viral

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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