| alphaB-crystallin suppresses oxidative stress-induced astrocyte apoptosis by inhibiting caspase-3 activation. | |
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MedLine Citation:
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PMID: 19379782 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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alphaB-crystallin is a member of the small heat shock proteins, which is abundantly expressed in various vertebrate tissues including the central nervous system. In our previous report, we showed alphaB-crystallin induction in activated astrocytes in the postischemic brain and in H2O2-treated primary astrocyte cultures. To investigate the functional significance of alphaB-crystallin induction in astrocytes, we generated a stable C6 astroglioma cell line overexpressing alphaB-crystallin. In these cells, hydrogen peroxide-induced apoptosis was reduced by 60% compared to parent cells. Furthermore, the repression of alphaB-crystallin expression by alphaB-crystallin siRNA transfection suppressed this protective effect, indicating that alphaB-crystallin is responsible for the protection against H2O2-induced apoptosis in C6 astroglioma cells. Similar level of aggravation in H2O2-induced apoptosis was observed in primary astrocyte cultures when alphaB-crystallin expression was suppressed by alphaB-crystallin siRNA transfection, confirming the importance of alphaB-crystallin. In addition, the induction of caspase-3 activity after H2O2 treatment was markedly suppressed in alphaB-crystallin-overexpressing cells, and immunoprecipitation proved binding between alphaB-crystallin and partially processed caspase-3 (a p24 intermediate). These results indicate that alphaB-crystallin confers protection against hydrogen peroxide-induced astrocytes apoptosis in part by inhibiting caspase-3 activation. |
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Authors:
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Joo-Hyun Shin; Seung-Woo Kim; Chae-Moon Lim; Ji-Young Jeong; Chun-Shu Piao; Ja-Kyeong Lee |
Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2009-04-18 |
Journal Detail:
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Title: Neuroscience research Volume: 64 ISSN: 1872-8111 ISO Abbreviation: Neurosci. Res. Publication Date: 2009 Aug |
Date Detail:
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Created Date: 2009-06-16 Completed Date: 2009-12-03 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 8500749 Medline TA: Neurosci Res Country: Ireland |
Other Details:
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Languages: eng Pagination: 355-61 Citation Subset: IM |
Affiliation:
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Department of Anatomy, Inha University School of Medicine, Inchon, Republic of Korea. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Animals Apoptosis / drug effects, physiology* Astrocytes / drug effects, metabolism* Caspase 3 / metabolism* Cell Line, Tumor Cytoprotection / physiology Down-Regulation / physiology Hydrogen Peroxide / toxicity Neurodegenerative Diseases / genetics, metabolism, physiopathology Oxidants / toxicity Oxidative Stress / drug effects, physiology* Protein Binding / physiology RNA Interference / physiology Rats Transfection alpha-Crystallin B Chain / genetics*, metabolism* |
| Chemical | |
Reg. No./Substance:
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0/Oxidants; 0/alpha-Crystallin B Chain; 7722-84-1/Hydrogen Peroxide; EC 3.4.22.-/Caspase 3 |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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