| alpha4-Integrin(+) endothelium derived from primate embryonic stem cells generates primitive and definitive hematopoietic cells. | |
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MedLine Citation:
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PMID: 17090646 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The mechanism of commencement of hematopoiesis in blood islands of the yolk sac and the aorta-gonad-mesonephros (AGM) region during primate embryogenesis remains elusive. In this study, we demonstrated that VE-cadherin(+)CD45(-) endothelial cells derived from nonhuman primate embryonic stem cells are able to generate primitive and definitive hematopoietic cells sequentially, as revealed by immunostaining of floating erythrocytes and colony-forming assay in cultures. Single bipotential progenitors for hematopoietic and endothelial lineages are included in this endothelial cell population. Furthermore, hemogenic activity of these endothelial cells is observed exclusively in the alpha4-integrin(+) subpopulation; bipotential progenitors are 4-fold enriched in this subpopulation. The kinetics of this hemogenic subpopulation is similar to that of hemogenic endothelial cells previously reported in the yolk sac and the AGM region in vivo in that they emerge for only a limited time. We suggest that VE-cadherin(+)CD45(-)alpha4-integrin(+) endothelial cells are involved in primitive and definitive hematopoiesis during primate embryogenesis, though VE-cadherin(-)CD45(-)alpha4-integrin(+) cells are the primary sources for primitive hematopoiesis. |
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Authors:
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Gen Shinoda; Katsutsugu Umeda; Toshio Heike; Masato Arai; Akira Niwa; Feng Ma; Hirofumi Suemori; Hong Yuan Luo; David H K Chui; Ryuzo Torii; Masabumi Shibuya; Norio Nakatsuji; Tatsutoshi Nakahata |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2006-11-07 |
Journal Detail:
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Title: Blood Volume: 109 ISSN: 0006-4971 ISO Abbreviation: Blood Publication Date: 2007 Mar |
Date Detail:
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Created Date: 2007-03-07 Completed Date: 2007-04-02 Revised Date: 2007-11-15 |
Medline Journal Info:
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Nlm Unique ID: 7603509 Medline TA: Blood Country: United States |
Other Details:
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Languages: eng Pagination: 2406-15 Citation Subset: AIM; IM |
Affiliation:
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Department of Pediatrics, Graduate School of Medicine, Kyoto University, Japan. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Animals Antigens, CD / metabolism Antigens, CD45 / metabolism Cadherins / metabolism Cells, Cultured Embryonic Stem Cells / cytology*, metabolism* Endothelial Cells / cytology, metabolism Endothelium / metabolism Erythrocytes / cytology, metabolism Hematopoiesis* Integrin alpha4 / metabolism* Macaca fascicularis |
| Chemical | |
Reg. No./Substance:
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0/Antigens, CD; 0/Cadherins; 0/cadherin 5; 143198-26-9/Integrin alpha4; EC 3.1.3.48/Antigens, CD45 |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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