| The alpha isoform of protein kinase C mediates phorbol ester-induced growth inhibition and p21cip1 induction in HC11 mammary epithelial cells. | |
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MedLine Citation:
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PMID: 10597271 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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To clarify the roles of specific isoforms of PKC in regulating growth and cell cycle progression of the HC11 mammary epithelial cell line, we investigated the effects of activating endogenous PKC isoforms with the phorbol ester tumor promoter TPA, and also the effects of TPA on genetically engineered cells containing increased levels of individual PKC isoforms. We found that TPA treatment of HC11 cells induced a transient cell cycle arrest in G0/G1. Western blot analyses of the TPA treated cells provided evidence that the endogenous PKC alpha present in these cells mediated these effects. Indeed, derivatives of the HC11 cell line that inducibly overexpress an exogenous PKC alpha or ectopic PKC beta 1 exhibited more marked growth inhibition by TPA than control cells. Immunohistochemical staining of cells following treatment with TPA revealed selective translocation of PKC alpha into the nucleus, whereas PKC beta 1 remained in the cytoplasm. The transient arrest of HC11 cells following treatment with TPA was associated with marked induction of both p21cip1 mRNA and protein. This induction was exaggerated in the derivatives that overexpressed either PKC alpha or PKC beta 1. Therefore, in mouse mammary epithelial cells activation of the endogenous PKC alpha can transiently arrest cells in G0/G1 which may be due, at least in part, to induction of the transcription of p21cip1. |
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Authors:
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E D Slosberg; M G Klein; Y Yao; E K Han; I Schieren; I B Weinstein |
Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S. |
Journal Detail:
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Title: Oncogene Volume: 18 ISSN: 0950-9232 ISO Abbreviation: Oncogene Publication Date: 1999 Nov |
Date Detail:
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Created Date: 2000-01-06 Completed Date: 2000-01-06 Revised Date: 2012-06-25 |
Medline Journal Info:
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Nlm Unique ID: 8711562 Medline TA: Oncogene Country: ENGLAND |
Other Details:
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Languages: eng Pagination: 6658-66 Citation Subset: IM |
Affiliation:
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Herbert Irving Comprehensive Cancer Center, Columbia University, New York, NY 10032, USA. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Animals Breast / cytology, drug effects*, enzymology, metabolism Cell Cycle Cell Division / drug effects* Cell Line Cyclin-Dependent Kinase Inhibitor p21 Cyclins / biosynthesis* Epithelial Cells / enzymology, metabolism Humans Isoenzymes / metabolism* Mice Protein Kinase C / metabolism* Protein Kinase C-alpha Tetradecanoylphorbol Acetate / pharmacology* Thymidine / metabolism |
| Grant Support | |
ID/Acronym/Agency:
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CA02656/CA/NCI NIH HHS; T32CA09503/CA/NCI NIH HHS; T32GM07088/GM/NIGMS NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/CDKN1A protein, human; 0/Cdkn1a protein, mouse; 0/Cyclin-Dependent Kinase Inhibitor p21; 0/Cyclins; 0/Isoenzymes; 16561-29-8/Tetradecanoylphorbol Acetate; 50-89-5/Thymidine; EC 2.7.11.13/PRKCA protein, human; EC 2.7.11.13/Prkca protein, mouse; EC 2.7.11.13/Protein Kinase C; EC 2.7.11.13/Protein Kinase C-alpha |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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