Document Detail


The alpha catalytic subunit of protein kinase CK2 is required for mouse embryonic development.
MedLine Citation:
PMID:  17954558     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Protein kinase CK2 (formerly casein kinase II) is a highly conserved and ubiquitous serine/threonine kinase that is composed of two catalytic subunits (CK2alpha and/or CK2alpha') and two CK2beta regulatory subunits. CK2 has many substrates in cells, and key roles in yeast cell physiology have been uncovered by introducing subunit mutations. Gene-targeting experiments have demonstrated that in mice, the CK2beta gene is required for early embryonic development, while the CK2alpha' subunit appears to be essential only for normal spermatogenesis. We have used homologous recombination to disrupt the CK2alpha gene in the mouse germ line. Embryos lacking CK2alpha have a marked reduction in CK2 activity in spite of the presence of the CK2alpha' subunit. CK2alpha(-/-) embryos die in mid-gestation, with abnormalities including open neural tubes and reductions in the branchial arches. Defects in the formation of the heart lead to hydrops fetalis and are likely the cause of embryonic lethality. Thus, CK2alpha appears to play an essential and uncompensated role in mammalian development.
Authors:
David Y Lou; Isabel Dominguez; Paul Toselli; Esther Landesman-Bollag; Conor O'Brien; David C Seldin
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Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't     Date:  2007-10-22
Journal Detail:
Title:  Molecular and cellular biology     Volume:  28     ISSN:  1098-5549     ISO Abbreviation:  Mol. Cell. Biol.     Publication Date:  2008 Jan 
Date Detail:
Created Date:  2007-12-19     Completed Date:  2008-01-11     Revised Date:  2009-11-19    
Medline Journal Info:
Nlm Unique ID:  8109087     Medline TA:  Mol Cell Biol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  131-9     Citation Subset:  IM    
Affiliation:
Boston University Medical Center, 650 Albany Street, Boston, MA 02118, USA.
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MeSH Terms
Descriptor/Qualifier:
Animals
Biological Markers
Casein Kinase II / chemistry,  deficiency,  genetics,  metabolism*
Catalytic Domain
Cell Differentiation
Cells, Cultured
Embryo, Mammalian / cytology,  embryology*,  metabolism*
Gene Expression Regulation, Developmental*
Gene Targeting
Heart / embryology
Mice
Mice, Knockout
Myocardium / metabolism
Protein Subunits / chemistry,  deficiency,  genetics,  metabolism
RNA, Messenger / genetics
Transcription, Genetic
Grant Support
ID/Acronym/Agency:
P01 ES011624/ES/NIEHS NIH HHS; R01 CA71796/CA/NCI NIH HHS; T32 CA064070/CA/NCI NIH HHS
Chemical
Reg. No./Substance:
0/Biological Markers; 0/Protein Subunits; 0/RNA, Messenger; EC 2.7.11.1/Casein Kinase II
Comments/Corrections

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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