Document Detail

An alpha-actinin isoform which may cross-link intermediate filaments and microfilaments.
MedLine Citation:
PMID:  9853368     Owner:  NLM     Status:  MEDLINE    
The G.3.5 antigen (named for the monoclonal antibody which recognizes it) has been characterized as an intermediate filament-associated protein found in a variety of tissue types, including human and rat astrocytes, rat skeletal and cardiac myocytes, fibroblasts, rat hepatocytes, and chicken and fish retinal tissues. Sequencing of proteolytic fragments indicated a high degree of similarity to alpha-actinin. Comparison of the G.3.5 antigen to alpha-actinin revealed that alpha-actinin and the G.3.5 antigen migrated similarly in reducing and non-reducing environments and had similar molecular masses (approximately 100,000). Overlay-immunoblotting assays indicated that the G.3.5 antigen and alpha-actinin could bind filamentous actin and desmin simultaneously. In contrast, immunocytochemistry indicated the G.3.5 antigen and alpha-actinin were immunologically distinct in tissue sections. The results of this study suggest that the G.3.5 antigen is an isoform of alpha-actinin which may serve to cross-link intermediate filaments to microfilaments, and that other isoforms of alpha-actinin may also share this property.
S H Bolanos; D O Zamora; D M García; J R Koke
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Publication Detail:
Type:  Comparative Study; Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, Non-P.H.S.    
Journal Detail:
Title:  Cytobios     Volume:  94     ISSN:  0011-4529     ISO Abbreviation:  Cytobios     Publication Date:  1998  
Date Detail:
Created Date:  1999-02-26     Completed Date:  1999-02-26     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0207227     Medline TA:  Cytobios     Country:  ENGLAND    
Other Details:
Languages:  eng     Pagination:  39-61     Citation Subset:  IM    
Department of Biology, Southwest Texas State University, San Marcos 78666, USA.
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MeSH Terms
Actinin / chemistry,  immunology,  isolation & purification,  metabolism*
Amino Acid Sequence
Antibodies, Monoclonal / immunology
Antibody Specificity
In Situ Hybridization
Intermediate Filament Proteins / metabolism*
Intermediate Filaments / metabolism*,  ultrastructure
Microfilaments / metabolism*,  ultrastructure
Molecular Sequence Data
Molecular Weight
Muscle Proteins / chemistry,  immunology,  metabolism*
Muscle, Skeletal / chemistry
Muscle, Smooth / chemistry
Protein Isoforms / chemistry,  immunology,  metabolism*
Sequence Homology, Amino Acid
Reg. No./Substance:
0/Antibodies, Monoclonal; 0/Intermediate Filament Proteins; 0/Muscle Proteins; 0/Protein Isoforms; 11003-00-2/Actinin

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