Document Detail


alpha-Toxin of Staphylococcus aureus overcomes acquired cisplatin-resistance in malignant mesothelioma cells.
MedLine Citation:
PMID:  18362050     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
alpha-Toxin (alpha-hemolysin) of Staphylococcus aureus is a pore-forming bacterial toxin which after caveolin-1-dependent assembly induces apoptosis in eukaryotic cells. We investigated if a sub-toxic concentration of staphylococcal alpha-toxin could enhance cisplatin-induced apoptosis and overcome acquired cisplatin-resistance in cultured malignant pleural mesothelioma (MPM) cells. MPM cells (P31wt) and a cisplatin-resistant sub-line (P31res) was incubated with alpha-toxin and/or cisplatin followed by determination of cell viability, apoptosis, and signaling pathways. P31res cells were more sensitive to alpha-toxin than P31 wt cells due to induction of apoptosis. A low-toxic concentration of alpha-toxin re-sensitized cisplatin P31res cytotoxicity by apoptosis-induced through the mitochondrial pathway without detectable activation of common up-stream apoptosis signaling proteins. The toxin/drug combination should be tested for cisplatin-resistant mesothelioma treatment.
Authors:
D Johansson; A Johansson; Parviz Behnam-Motlagh
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2008-03-24
Journal Detail:
Title:  Cancer letters     Volume:  265     ISSN:  0304-3835     ISO Abbreviation:  Cancer Lett.     Publication Date:  2008 Jun 
Date Detail:
Created Date:  2008-05-19     Completed Date:  2008-09-02     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  7600053     Medline TA:  Cancer Lett     Country:  Ireland    
Other Details:
Languages:  eng     Pagination:  67-75     Citation Subset:  IM    
Affiliation:
Department of Medical Biosciences, Clinical Chemistry, Umeå University, Building 6M, 2nd Floor, S-901 85 Umeå, Sweden. david.johansson@medbio.umu.se
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MeSH Terms
Descriptor/Qualifier:
Antineoplastic Agents / pharmacology*
Apoptosis
Bacterial Toxins / pharmacology*
Cell Line, Tumor
Cell Survival / drug effects
Cisplatin / pharmacology*
Drug Resistance, Neoplasm / drug effects*
Hemolysin Proteins / pharmacology*
Humans
Mesothelioma
Pleural Neoplasms
Signal Transduction
Staphylococcus aureus*
Chemical
Reg. No./Substance:
0/Antineoplastic Agents; 0/Bacterial Toxins; 0/Hemolysin Proteins; 0/staphylococcal alpha-toxin; 15663-27-1/Cisplatin

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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