| An α-linolenic acid-rich formula reduces oxidative stress and inflammation by regulating NF-κB in rats with TNBS-induced colitis. | |
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MedLine Citation:
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PMID: 20724486 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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We have previously shown that α-linolenic acid (ALA), a (n-3) PUFA exerts in vitro antiinflammatory effects in the intestine. In this study, we aimed to evaluate its effect on inflammatory and oxidative stress in a colitis model. Colitis was induced in 2 groups at d 0 by intrarectal injection of 2-4-6-trinitrobenzen sulfonic acid (TNBS), whereas the control group received the vehicle. Rats we fed 450 mg . kg(-1) . d(-1) of ALA (TNBS+ALA) while the other colitic group (TNBS) and the control group were fed an isocaloric corn oil formula for 14 d (from d -7 to d 7). RBC fatty acid composition was assessed. Oxidative stress was studied by measuring urinary 8-isoprostanes (8-IP) and colon glutathione (GSH) concentration and inducible nitric oxide synthase (iNOS) expression. Colitis was assessed histologically, by production of proinflammatory mediators, including cytokines, leukotrienes B(4) (LTB(4)), and cyclooxygenase-2 (COX-2) and by nuclear factor-κB (NF-κB) activation. The ALA-rich diet significantly increased the RBC levels of ALA, eicosapentaenoic acid, and docosapentaenoic acid (n-3) compared with the TNBS group (P < 0.01 for all). The beneficial effect of ALA supplementation on oxidative stress was reflected by lower urinary 8-IP levels (P < 0.05), a normalized colon GSH concentration (P < 0.01), and reduced colon iNOS expression (P < 0.05) compared with the TNBS group. ALA also protected against colon inflammation as assessed by lower tumor necrosis factor-α secretion and mRNA level (P < 0.05), reduced NF-κB activation (P = 0.01), and lower colon lipid mediator concentrations such as LTB(4) and COX-2 (P < 0.05) compared with the TNBS group. These findings show that an ALA-rich formula is beneficial to TNBS-induced colitic rats via inhibition of oxidative and inflammatory stress. |
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Authors:
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Aktham Hassan; Ayman Ibrahim; Khaly Mbodji; Moïse Coëffier; Frédéric Ziegler; Frédéric Bounoure; Jean-Michel Chardigny; Mohamed Skiba; Guillaume Savoye; Pierre Déchelotte; Rachel Marion-Letellier |
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Publication Detail:
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Type: Journal Article Date: 2010-08-19 |
Journal Detail:
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Title: The Journal of nutrition Volume: 140 ISSN: 1541-6100 ISO Abbreviation: J. Nutr. Publication Date: 2010 Oct |
Date Detail:
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Created Date: 2010-09-21 Completed Date: 2010-10-19 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 0404243 Medline TA: J Nutr Country: United States |
Other Details:
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Languages: eng Pagination: 1714-21 Citation Subset: IM |
Affiliation:
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Appareil Digestif Environnement Nutrition, Medicine University, I.F.R. 23, Institute of biomedical research, 22, 76183 Rouen cedex, France. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Animals Chymotrypsin / metabolism Colitis / chemically induced, metabolism*, prevention & control* Colon / chemistry, metabolism, pathology Cytokines / analysis Diet Dinoprost / analogs & derivatives, urine Eicosanoids / biosynthesis Erythrocytes / chemistry Fatty Acids / blood Glutathione / analysis Interferons / analysis Male NF-kappa B / analysis, drug effects*, physiology Nitric Oxide Synthase Type II / analysis Oxidative Stress / drug effects* Rats Rats, Sprague-Dawley Trinitrobenzenesulfonic Acid* / administration & dosage alpha-Linolenic Acid / administration & dosage* |
| Chemical | |
Reg. No./Substance:
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0/Cytokines; 0/Eicosanoids; 0/Fatty Acids; 0/NF-kappa B; 2508-19-2/Trinitrobenzenesulfonic Acid; 27415-26-5/8-epi-prostaglandin F2alpha; 463-40-1/alpha-Linolenic Acid; 551-11-1/Dinoprost; 70-18-8/Glutathione; 9008-11-1/Interferons; EC 1.14.13.39/Nitric Oxide Synthase Type II; EC 3.4.21.1/Chymotrypsin |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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