Document Detail

The alpha-D-glucosyl C-2 hydroxyl is required for binding to the H(+)-sucrose transporter in phloem.
MedLine Citation:
PMID:  8399306     Owner:  NLM     Status:  MEDLINE    
The specificity of uptake of sucrose into isolated phloem tissue from Apium graveolens has been investigated using a number of analogues of sucrose. The presence of a single saturable transport system for sucrose was confirmed using the double isotope method of Inui and Christensen (J. Gen. Physiol. 50 (1966) 203-224). 4-Hydroxyphenyl beta-D-fructofuranoside showed no inhibition of sucrose uptake, whereas 4-hydroxyphenyl alpha-D-glucopyranoside showed competitive inhibition with a Ki of 6.7 mM. 4-Methoxyphenyl alpha-D-glucopyranoside also inhibited sucrose uptake competitively (Ki = 6.0 mM). This compound was also synthesised radioactively labelled with 14C and its uptake into phloem tissue was conclusively demonstrated to occur by active transport on the same carrier as sucrose. Contrastingly, 4-methoxyphenyl alpha-D-2-deoxyglucopyranoside displayed non-competitive inhibition of sucrose influx (Ki = 2.5 mM) and uptake of the 14C-labelled compound was insensitive to FCCP, PCMBS and sucrose. We conclude that the hydroxyl group at the C-2 position on the glucopyranosyl moiety is essential for binding to the sucrose carrier in this tissue.
S D Griffin; K D Buxton; I A Donaldson
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Biochimica et biophysica acta     Volume:  1152     ISSN:  0006-3002     ISO Abbreviation:  Biochim. Biophys. Acta     Publication Date:  1993 Oct 
Date Detail:
Created Date:  1993-11-09     Completed Date:  1993-11-09     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0217513     Medline TA:  Biochim Biophys Acta     Country:  NETHERLANDS    
Other Details:
Languages:  eng     Pagination:  61-8     Citation Subset:  IM    
Department of Biochemistry, University of Oxford, UK.
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MeSH Terms
Arbutin / chemical synthesis,  metabolism
Binding, Competitive
Biological Transport, Active
Carbon Radioisotopes
Carbonyl Cyanide p-Trifluoromethoxyphenylhydrazone
Carrier Proteins / metabolism*
Cell Membrane / metabolism
Glycosides / chemical synthesis,  metabolism
Phenols / chemical synthesis,  metabolism
Plant Proteins / metabolism*
Sucrose / analogs & derivatives,  metabolism*
Reg. No./Substance:
0/Carbon Radioisotopes; 0/Carrier Proteins; 0/Glycosides; 0/Phenols; 0/Plant Proteins; 370-86-5/Carbonyl Cyanide p-Trifluoromethoxyphenylhydrazone; 497-76-7/Arbutin; 554-77-8/4-Chloromercuribenzenesulfonate; 57-50-1/Sucrose; 95593-76-3/4-hydroxyphenyl beta-fructofuranoside

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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