Document Detail


alpha-Amino-n-butyric acid methyl ester induces concentrative uptake of L-dopa in human Langerhans' cells normally not operative for L-dopa transport.
MedLine Citation:
PMID:  8105618     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
We recently reported the existence of two kinds of human epidermal Langerhans' cells (LC), one which can take up and accumulate L-dopa and one which cannot. The dopa(+) LC take up L-dopa by carrier-mediated exchange diffusion, that is, the influx of L-dopa and the outflow of an intracellular substance are linked via the same carrier. The nature of the fundamental difference between L-dopa(+) and L-dopa(-) cells has not been clarified. We have now found that alpha-amino-n-butyric acid methyl ester (ABA-OME) penetrates into intracellular compartments, perhaps endosomes or lysosomes, of all LC, where hydrolysis results in the accumulation of the free amino acid (ABA). This accumulation causes a considerable increase in osmotic pressure of the membrane-limited organelle, leading to influx of water and swelling. Co-incubation with L-dopa revealed an influx of L-dopa into LC which normally cannot take up this amino acid. It is suggested that these LC lack the capacity to synthesize and/or store the counterpart which allows L-dopa to enter the dopa(+) LC, but that ABA in the L-dopa(-) LC can function as an equivalent counterpart.
Authors:
B Falck; L Andersson; A Mikulowska; G Ronquist
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Publication Detail:
Type:  In Vitro; Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Acta dermato-venereologica     Volume:  73     ISSN:  0001-5555     ISO Abbreviation:  Acta Derm. Venereol.     Publication Date:  1993 Jun 
Date Detail:
Created Date:  1993-11-23     Completed Date:  1993-11-23     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0370310     Medline TA:  Acta Derm Venereol     Country:  SWEDEN    
Other Details:
Languages:  eng     Pagination:  197-9     Citation Subset:  IM    
Affiliation:
Department of Medical Cell Research, University of Lund, Sweden.
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MeSH Terms
Descriptor/Qualifier:
Adult
Aminobutyric Acids / pharmacology*
Biological Transport / drug effects
Humans
Langerhans Cells / drug effects,  metabolism*,  ultrastructure
Levodopa / pharmacokinetics*
Chemical
Reg. No./Substance:
0/Aminobutyric Acids; 0/Levodopa; 2483-62-7/2-aminobutyric acid methyl ester

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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