Document Detail


alpha 2HS glycoprotein is chemotactic for mononuclear phagocytes.
MedLine Citation:
PMID:  3110177     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
alpha 2HS glycoprotein is a normal constituent of plasma. It has a high affinity for hydroxyapatite and is concentrated in bone 100-fold greater than albumin, however its biological function in bone is not known. We have purified alpha 2HS from human plasma and examined it for evidence of chemotactic activity against human peripheral mononuclear cells, polymorphonuclear leucocytes and lymphocytes in Boyden chamber assays. We observed that the protein was chemotactic for mononuclear cells and that maximal cell migration occurred when its concentration in the lower compartment of Boyden chambers was 10(-10) M. Chemotaxis did not occur in the absence of a concentration gradient. In addition, cell migration was blocked when the cells were preincubated with 10(-10) M alpha 2HS or when the protein was preincubated with rabbit anti-human alpha 2HS glycoprotein IgG. Neither polymorphonuclear leucocytes, which are responsive to a wide range of chemoattractants, or peripheral lymphocytes exhibited directed migration to alpha 2HS in Boyden chamber assays. The glycoprotein appears therefore to act as a chemotaxin directed to monocyte recruitment, and we speculate that the protein may have an important role in monocyte recruitment to bone and possibly their subsequent fusion and differentiation into functional osteoclasts.
Authors:
J D Malone; M Richards
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Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, Non-P.H.S.; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Journal of cellular physiology     Volume:  132     ISSN:  0021-9541     ISO Abbreviation:  J. Cell. Physiol.     Publication Date:  1987 Jul 
Date Detail:
Created Date:  1987-08-19     Completed Date:  1987-08-19     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  0050222     Medline TA:  J Cell Physiol     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  118-24     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Blood Proteins / pharmacology*
Chemotaxis, Leukocyte / drug effects*
Electrophoresis, Polyacrylamide Gel
Humans
Immunodiffusion
Monocytes
N-Formylmethionine Leucyl-Phenylalanine / pharmacology
Phagocytes
Grant Support
ID/Acronym/Agency:
AM-33961/AM/NIADDK NIH HHS
Chemical
Reg. No./Substance:
0/Blood Proteins; 0/alpha2HS glycoprotein; 59880-97-6/N-Formylmethionine Leucyl-Phenylalanine

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