| alpha 1-Fetoprotein mRNA of rat yolk sac and hepatoma. | |
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MedLine Citation:
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PMID: 91159 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Rat alpha 1-fetoprotein mRNA was isolated and purified to apparent homogeneity by means of immunoadsorption and oligo (dT) cellulose affinity chromatography. Purified AFP mRNA migrated as a 21S peak in 2.5% SDS-polyacrylamide gels. The translation product of this mRNA in micrococcal nuclease treated reticulocyte lysate was identified as AFP by specific immunoprecipitation, SDS-gel electrophoresis and tryptic digestion analysis. DNA complimentary to AFP mRNA was synthesized with avian meyloblastosis virus RNA-dependent DNA polymerase. This AFP cDNA was used as a probe to quantitate AFP mRNA in the developing rat liver and to compare the complexity and diversity of AFP mRNA derived from the normal rat liver and Morris hepatoma 7777. We found that the amount of functional AFP mRNA is decreasing during liver development. There is very little, if any, AFP mRNA in the adult rat liver. A high degree of homology between the AFP mRNA sequences of yolk sac and hepatoma was also found. |
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Authors:
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J F Chiu; W Decha-Umphai; P Commer |
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Publication Detail:
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Type: Journal Article; Research Support, U.S. Gov't, P.H.S. |
Journal Detail:
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Title: Nucleic acids research Volume: 7 ISSN: 0305-1048 ISO Abbreviation: Nucleic Acids Res. Publication Date: 1979 Sep |
Date Detail:
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Created Date: 1979-12-27 Completed Date: 1979-12-27 Revised Date: 2009-11-18 |
Medline Journal Info:
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Nlm Unique ID: 0411011 Medline TA: Nucleic Acids Res Country: ENGLAND |
Other Details:
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Languages: eng Pagination: 239-49 Citation Subset: IM |
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| MeSH Terms | |
Descriptor/Qualifier:
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Animals Base Sequence Female Liver / metabolism Liver Neoplasms, Experimental / metabolism* Micrococcal Nuclease Pregnancy Protein Biosynthesis RNA, Messenger* / isolation & purification, metabolism Rats Yolk Sac / metabolism* alpha-Fetoproteins / biosynthesis* |
| Chemical | |
Reg. No./Substance:
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0/RNA, Messenger; 0/alpha-Fetoproteins; EC 3.1.31.1/Micrococcal Nuclease |
| Comments/Corrections | |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
| Full Text | |
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Journal Information Journal ID (nlm-ta): Nucleic Acids Res ISSN: 0305-1048 ISSN: 1362-4962 |
Article Information Download PDF  Print publication date: Day: 11 Month: 9 Year: 1979 Volume: 7 Issue: 1 First Page: 239 Last Page: 249 ID: 328009 PubMed Id: 91159 |
| alpha 1-Fetoprotein mRNA of rat yolk sac and hepatoma. | |
| J F Chiu | |
| W Decha-Umphai | |
| P Commer | |
Article Categories:
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