| The alkaline single cell gel electrophoresis: a new test for assessing DNA single strand breaks in Neurospora crassa. | |
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MedLine Citation:
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PMID: 9729255 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The single cell gel electrophoresis (comet assay) is a potent technique in testing double and single strand breaks in DNA. In this paper, we present an application of alkaline comet assay to filamentous fungi for genotoxicological assessment of heavy metals for the first time. A wild strain of Neurospora crassa SLA 4200 was grown in presence of cadmium sulfate (CdSO4) (10 microM and 100 microM) for 12 h. Protoplasts from 12-h old mycelia were prepared by using Novozym 234 and DNA damage was evaluated by alkaline comet assay. Hydrogen peroxide (H2O2) (50 microM and 100 microM) was taken as an internal standard for DNA damage. Both CdSO4 and H2O2 induced significant single strand breaks in DNA. The results indicate that alkaline comet assay is a sensitive and rapid method for DNA damage analysis in filamentous fungal systems. |
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Authors:
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M Bhanoori; G Venkateswerlu |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Mutation research Volume: 405 ISSN: 0027-5107 ISO Abbreviation: Mutat. Res. Publication Date: 1998 Aug |
Date Detail:
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Created Date: 1998-10-02 Completed Date: 1998-10-02 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 0400763 Medline TA: Mutat Res Country: NETHERLANDS |
Other Details:
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Languages: eng Pagination: 29-34 Citation Subset: IM |
Copyright Information:
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Copyright 1998 Elsevier Science B.V. |
Affiliation:
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Department of Biochemistry, Osmania University, Hyderabad 500 007, India. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Cadmium
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pharmacology DNA / analysis DNA Damage / genetics* Electrophoresis / methods Hydrogen Peroxide / pharmacology Mutagenicity Tests Neurospora crassa / genetics* Protoplasts / drug effects |
| Chemical | |
Reg. No./Substance:
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7440-43-9/Cadmium; 7722-84-1/Hydrogen Peroxide; 9007-49-2/DNA |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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