| The alignment and fusion assembly of adipose-derived stem cells on mechanically patterned matrices. | |
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MedLine Citation:
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PMID: 22800539 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Cell patterning is typically accomplished by selectively depositing proteins for cell adhesion only on patterned regions; however in tissues, cells are also influenced by mechanical stimuli, which can also result in patterned arrangements of cells. We developed a mechanically-patterned hydrogel to observe and compare it to extracellular matrix (ECM) ligand patterns to determine how to best regulate and improve cell type-specific behaviors. Ligand-based patterning on hydrogels was not robust over prolonged culture, but cells on mechanically-patterned hydrogels differentially sorted based on stiffness preference: myocytes and adipose-derived stem cells (ASCs) underwent stiffness-mediated migration, i.e. durotaxis, and remained on myogenic hydrogel regions. Myocytes developed aligned striations and fused on myogenic stripes of the mechanically-patterned hydrogel. ASCs aligned and underwent myogenesis, but their fusion rate increased, as did the number of cells fusing into a myotube as a result of their alignment. Conversely, neuronal cells did not exhibit durotaxis and could be seen on soft regions of the hydrogel for prolonged culture time. These results suggest that mechanically-patterned hydrogels could provide a platform to create tissue engineered, innervated micro-muscles of neural and muscle phenotypes juxtaposed next to each other in order better recreate a muscle niche. |
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Authors:
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Yu Suk Choi; Ludovic G Vincent; Andrew R Lee; Kyle C Kretchmer; Somyot Chirasatitsin; Marek K Dobke; Adam J Engler |
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Publication Detail:
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Type: Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, Non-P.H.S. Date: 2012-07-15 |
Journal Detail:
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Title: Biomaterials Volume: 33 ISSN: 1878-5905 ISO Abbreviation: Biomaterials Publication Date: 2012 Oct |
Date Detail:
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Created Date: 2012-07-30 Completed Date: 2012-11-19 Revised Date: 2013-04-16 |
Medline Journal Info:
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Nlm Unique ID: 8100316 Medline TA: Biomaterials Country: England |
Other Details:
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Languages: eng Pagination: 6943-51 Citation Subset: IM |
Copyright Information:
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Copyright © 2012 Elsevier Ltd. All rights reserved. |
Affiliation:
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Departments of Bioengineering, University of California, San Diego, CA 92093, USA. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Adipose Tissue
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cytology* Adult Animals Biocompatible Materials / chemistry* Cell Culture Techniques / methods* Cell Differentiation Cell Lineage Cells, Cultured Chickens Humans Hydrogel Hydrogels / chemistry Mice Muscle Cells / cytology Muscle Fibers, Skeletal / cytology* Muscles / cytology Neurons / metabolism Phenotype Stem Cells / cytology* |
| Grant Support | |
ID/Acronym/Agency:
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DP02OD006460/OD/NIH HHS; DP2 OD006460/OD/NIH HHS; P30 AR061303/AR/NIAMS NIH HHS; P30AR061303/AR/NIAMS NIH HHS; R21 HL106529/HL/NHLBI NIH HHS; R21HL106529/HL/NHLBI NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/Biocompatible Materials; 0/Hydrogels; 25852-47-5/Hydrogel |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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