Document Detail


The alignment and fusion assembly of adipose-derived stem cells on mechanically patterned matrices.
MedLine Citation:
PMID:  22800539     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Cell patterning is typically accomplished by selectively depositing proteins for cell adhesion only on patterned regions; however in tissues, cells are also influenced by mechanical stimuli, which can also result in patterned arrangements of cells. We developed a mechanically-patterned hydrogel to observe and compare it to extracellular matrix (ECM) ligand patterns to determine how to best regulate and improve cell type-specific behaviors. Ligand-based patterning on hydrogels was not robust over prolonged culture, but cells on mechanically-patterned hydrogels differentially sorted based on stiffness preference: myocytes and adipose-derived stem cells (ASCs) underwent stiffness-mediated migration, i.e. durotaxis, and remained on myogenic hydrogel regions. Myocytes developed aligned striations and fused on myogenic stripes of the mechanically-patterned hydrogel. ASCs aligned and underwent myogenesis, but their fusion rate increased, as did the number of cells fusing into a myotube as a result of their alignment. Conversely, neuronal cells did not exhibit durotaxis and could be seen on soft regions of the hydrogel for prolonged culture time. These results suggest that mechanically-patterned hydrogels could provide a platform to create tissue engineered, innervated micro-muscles of neural and muscle phenotypes juxtaposed next to each other in order better recreate a muscle niche.
Authors:
Yu Suk Choi; Ludovic G Vincent; Andrew R Lee; Kyle C Kretchmer; Somyot Chirasatitsin; Marek K Dobke; Adam J Engler
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Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, Non-P.H.S.     Date:  2012-07-15
Journal Detail:
Title:  Biomaterials     Volume:  33     ISSN:  1878-5905     ISO Abbreviation:  Biomaterials     Publication Date:  2012 Oct 
Date Detail:
Created Date:  2012-07-30     Completed Date:  2012-11-19     Revised Date:  2013-10-17    
Medline Journal Info:
Nlm Unique ID:  8100316     Medline TA:  Biomaterials     Country:  England    
Other Details:
Languages:  eng     Pagination:  6943-51     Citation Subset:  IM    
Copyright Information:
Copyright © 2012 Elsevier Ltd. All rights reserved.
Affiliation:
Departments of Bioengineering, University of California, San Diego, CA 92093, USA.
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MeSH Terms
Descriptor/Qualifier:
Adipose Tissue / cytology*
Adult
Animals
Biocompatible Materials / chemistry*
Cell Culture Techniques / methods*
Cell Differentiation
Cell Lineage
Cells, Cultured
Chickens
Humans
Hydrogel
Hydrogels / chemistry
Mice
Muscle Cells / cytology
Muscle Fibers, Skeletal / cytology*
Muscles / cytology
Neurons / metabolism
Phenotype
Stem Cells / cytology*
Grant Support
ID/Acronym/Agency:
DP02OD006460/OD/NIH HHS; DP2 OD006460/OD/NIH HHS; P30 AR061303/AR/NIAMS NIH HHS; P30AR061303/AR/NIAMS NIH HHS; R21 HL106529/HL/NHLBI NIH HHS; R21HL106529/HL/NHLBI NIH HHS
Chemical
Reg. No./Substance:
0/Biocompatible Materials; 0/Hydrogels; 25852-47-5/Hydrogel
Comments/Corrections

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