Document Detail

The aldehyde fuchsin and colloidal iron staining reactions in the canine thyroid C cell.
MedLine Citation:
PMID:  80395     Owner:  NLM     Status:  MEDLINE    
The cytochemically reactive groups which are responsible for Aldehyde Fuchsin (AF) and colloidal iron (CI) staining of C cells were investigated in the canine thyroid gland. To this end, stains for proteoglycans and peptide groups were utilized in conjuction with hydrolysis of glycosidic and amide bonds. In addition, the following procedures were used: acetylation, benzoylation, nitrozation, aldehyde blockade, sulphydryl blockade, methylation and mild acid hydrolysis. No acidic proteoglycan, sialic acid, polyphosphate or polysaccharide ester sulphate were detected in C cells; the results suggest that AF staining, after an oxidation step, and CI staining are due to polypeptides. Sulphydryl and carboxyl groups together are necessary for mediating the attachment of AF in C cells and it is adduced that this attachment is due to the combined charges of sulphonic and carboxylic acids. Methylation and acetylation inhibit CI staining and those staining reactions that depend upon carboxylic acid (TB) and hydroxyl groups (PAS) for their dye attachment in C cells. Acid hydrolysis, which increases the demonstration of carboxylic acid in C cells, decreases the attachment of hydroxyferric ions. I speculate that this inhibition is due to extraction of iron sites in the C cell and conclude that it is not solely carboxylic acids in C cells that are responsible for CI staining.
W E Roediger
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Publication Detail:
Type:  Comparative Study; Journal Article    
Journal Detail:
Title:  The Histochemical journal     Volume:  10     ISSN:  0018-2214     ISO Abbreviation:  Histochem. J.     Publication Date:  1978 Sep 
Date Detail:
Created Date:  1978-12-02     Completed Date:  1978-12-02     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0163161     Medline TA:  Histochem J     Country:  ENGLAND    
Other Details:
Languages:  eng     Pagination:  549-57     Citation Subset:  IM    
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MeSH Terms
Proteoglycans / analysis*
Rosaniline Dyes*
Staining and Labeling / methods*
Thyroglobulin / analysis*
Thyroid Gland / analysis*
Reg. No./Substance:
0/Colloids; 0/Proteoglycans; 0/Rosaniline Dyes; 7439-89-6/Iron; 9010-34-8/Thyroglobulin

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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