Document Detail


An albumin-coated polyester arterial graft: in vivo assessment of biocompatibility and healing characteristics.
MedLine Citation:
PMID:  8962944     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The albumin-coated vascular graft (ACG) and its uncoated polyester substrate, the Vascular II (V-II), were evaluated in terms of biocompatibility and biofunctionality using two in vivo animal studies. Biocompatibility and immunoreactivity were assessed by implanting intraperitoneally in the rat small segments of the ACG and the V-II graft and harvesting them with their surrounding tissue 3d, 1, 2 and 4 weeks later. Cytofluorometric determination of total T cells (CD3), the ratio of CD4/CD8 subsets and the percentage of IL-2 receptor-positive T cells in the peripheral blood has revealed that no significant difference in any of the T cell populations was found between the ACG and the V-II graft. The cellular reactivity of the ACG in terms of acid phosphatase activity at the implant side was significantly greater at 3 d but not at longer periods. Biofunctionality was evaluated by implanting both grafts as a thoracoabdominal vascular bypass in dogs for 11 different periods ranging from 4 h to 6 months. The rate of albumin resorption was such that traces were still present at 1 month, but no longer observable at 2 months. Tissue incorporation into the graft wall was earlier for the V-II (2 weeks) than for the ACG (4 weeks), which showed complete encapsulation, tissue incorporation and endothelialization after 2 months in vivo. Only small differences were observed between both grafts in terms of platelet and fibrin uptake on the luminal surface. The prostacyclin/thromboxane A2 ratio increased to a level higher that 1.0 aorta within 1 month for the V-II and 4 months for the ACG. In conclusion, the Bard ACG has demonstrated excellent biocompatibility in terms of blood T cell behaviour and acid phosphatase activity at the implant site. Finally, its healing response is equivalent to that of the uncoated Dacron prosthesis once the albumin coating has been resorbed.
Authors:
Y Marois; N Chakfé; R Guidoin; R C Duhamel; R Roy; M Marois; M W King; Y Douville
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Biomaterials     Volume:  17     ISSN:  0142-9612     ISO Abbreviation:  Biomaterials     Publication Date:  1996 Jan 
Date Detail:
Created Date:  1996-12-12     Completed Date:  1996-12-12     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  8100316     Medline TA:  Biomaterials     Country:  ENGLAND    
Other Details:
Languages:  eng     Pagination:  3-14     Citation Subset:  IM; X    
Affiliation:
Department of Surgery, Medicine and Pathology, Laval University, Quebec, Canada.
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MeSH Terms
Descriptor/Qualifier:
Acid Phosphatase / metabolism
Albumins / chemistry*,  metabolism
Analysis of Variance
Angiography
Animals
Biocompatible Materials
Blood Platelets / metabolism
Blood Vessel Prosthesis / standards*
CD4-CD8 Ratio
Dogs
Epoprostenol / metabolism
Female
Fibrinogen / metabolism
Flow Cytometry
Glutaral / chemistry
Isotope Labeling
Microscopy, Electron, Scanning
Peritoneum
Polyesters / chemistry,  metabolism*
Prostheses and Implants
Rats
Rats, Sprague-Dawley
Receptor-CD3 Complex, Antigen, T-Cell / metabolism
Receptors, Interleukin-2 / metabolism
T-Lymphocytes / cytology,  enzymology
Thromboxane A2 / metabolism
Chemical
Reg. No./Substance:
0/Albumins; 0/Biocompatible Materials; 0/Polyesters; 0/Receptor-CD3 Complex, Antigen, T-Cell; 0/Receptors, Interleukin-2; 111-30-8/Glutaral; 35121-78-9/Epoprostenol; 57576-52-0/Thromboxane A2; 9001-32-5/Fibrinogen; EC 3.1.3.2/Acid Phosphatase

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