| An affinity column for the purification of prenyltransferases. | |
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MedLine Citation:
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PMID: 3907410 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Farnesyl pyrophosphate synthetase (EC 2.5.1.1) from chicken liver, pig liver, and yeast has been purified to homogeneity in a single chromatographic step by affinity chromatography. The affinity ligand, geranylmethylphosphonophosphate, is linked to Affi-Gel 10 through the phosphonophosphate moiety. The affinity gel is stable chemically and the internal phosphonophosphate linkage is not hydrolyzed by nonspecific phosphatases. A single column has been used repeatedly for over a year with no degradation in its performance. A typical purification only requires 2 days and gives a 500- to 600-fold purification of enzyme from a crude ammonium sulfate precipitate. |
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Authors:
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D L Bartlett; C H King; C D Poulter |
Publication Detail:
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Type: Journal Article; Research Support, U.S. Gov't, P.H.S. |
Journal Detail:
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Title: Analytical biochemistry Volume: 149 ISSN: 0003-2697 ISO Abbreviation: Anal. Biochem. Publication Date: 1985 Sep |
Date Detail:
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Created Date: 1986-01-08 Completed Date: 1986-01-08 Revised Date: 2007-11-14 |
Medline Journal Info:
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Nlm Unique ID: 0370535 Medline TA: Anal Biochem Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 507-15 Citation Subset: IM |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Affinity Labels Animals Chickens Chromatography, Affinity Dimethylallyltranstransferase / isolation & purification* Electrophoresis, Polyacrylamide Gel Ligands Liver / enzymology Saccharomyces cerevisiae / enzymology Swine Transferases / isolation & purification* |
| Grant Support | |
ID/Acronym/Agency:
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GM 09198/GM/NIGMS NIH HHS; GM 21328/GM/NIGMS NIH HHS; GM 25521/GM/NIGMS NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/Affinity Labels; 0/Ligands; EC 2.-/Transferases; EC 2.5.1.1/Dimethylallyltranstransferase |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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