Document Detail


The adrenal secretion of progesterone stimulates testicular steroidogenesis in the rat in vitro.
MedLine Citation:
PMID:  3001409     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
We have used a multicolumn isolated cell superfusion system to investigate the interaction between isolated rat adrenal and testicular cells in the secretion of sex steroids. The steroid responses of a mixed population of adrenal and testicular cells to the administration of ACTH 100 pg/ml was compared with the responses of the separate cell types. Steroid responses from 5 separate experiments were analysed. We have demonstrated increased secretion of 17 alpha-hydroxyprogesterone (P less than 0.0005), androstenedione (P less than 0.05) and testosterone (P less than 0.0005) by a mixture of cells when compared with the responses of either cell type alone. In contrast, the secretion of progesterone (P less than 0.0005) and corticosterone (P less than 0.005) was reduced in the mixed cell population, suggesting that progesterone is preferentially converted by testicular cells to 17 alpha-hydroxyprogesterone, androstenedione and testosterone. This is confirmed by increased secretion of 17 alpha-hydroxyprogesterone, androstenedione and testosterone but not corticosterone by a mixed population following the administration of progesterone 10 ng/ml. These results suggest that the adrenal secretion of progesterone can stimulate testicular steroidogenesis in the male rat in vitro.
Authors:
C M Feek; N L Tuzi; B C Williams; D Burt; F C Wu; A M Wallace; C E Gray; C R Edwards
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Publication Detail:
Type:  In Vitro; Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Journal of steroid biochemistry     Volume:  23     ISSN:  0022-4731     ISO Abbreviation:  J. Steroid Biochem.     Publication Date:  1985 Nov 
Date Detail:
Created Date:  1986-01-31     Completed Date:  1986-01-31     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0260125     Medline TA:  J Steroid Biochem     Country:  ENGLAND    
Other Details:
Languages:  eng     Pagination:  617-23     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Adrenal Glands / secretion*
Adrenocorticotropic Hormone / pharmacology
Animals
Chorionic Gonadotropin / pharmacology
Dose-Response Relationship, Drug
Gonadal Steroid Hormones / biosynthesis*
Male
Progesterone / pharmacology,  secretion*
Rats
Rats, Inbred Strains
Testis / metabolism*
Chemical
Reg. No./Substance:
0/Chorionic Gonadotropin; 0/Gonadal Steroid Hormones; 57-83-0/Progesterone; 9002-60-2/Adrenocorticotropic Hormone

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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