Document Detail


The adenovirus L3 23-kilodalton proteinase cleaves the amino-terminal head domain from cytokeratin 18 and disrupts the cytokeratin network of HeLa cells.
MedLine Citation:
PMID:  7684469     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Immunofluorescence studies revealed that adenovirus induces a reorganization of the cytokeratin system in lytically infected HeLa cells. At 24 h postinfection, the cytokeratin network began to disassemble into prominent spheroid globules. By 36 h postinfection, host cell lysis occurred, accompanied by the formation of perinuclear cytokeratin clumps and additional spheroid globules. Immunoblots detected 41- and 44-kDa fragments of cytokeratin 18 and reduced levels of cytokeratin 7 at 24 and 36 h postinfection. Cytokeratin proteins isolated from HeLa cells at 36 h postinfection were deficient in filament polymerization. The 41-kDa proteolytic cytokeratin 18-specific fragment was purified, and its amino-terminal sequence was determined to be GGIQNEKETM. These residues correspond to amino acids 74 through 83 of cytokeratin 18, identifying a cleavage site at the junction of the globular head domain and the alpha-helical rod domain. Moreover, this truncation event occurs at a consensus cleavage site for the adenovirus L3 23-kDa proteinase. The temperature-sensitive mutant H2-ts1, which contains a mutation in the proteinase, neither induced cleavage of cytokeratin 18 nor precipitated the formation of spheroid globules during lytic infection at the nonpermissive temperature. The active proteinase is therefore required for cleavage of cytokeratin 18 and morphological rearrangement of the cytokeratins. We suggest that disruptions in the cytokeratin system weaken the mechanical integrity of the cell, thus promoting host cell lysis and release of progeny virions.
Authors:
P H Chen; D A Ornelles; T Shenk
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Journal of virology     Volume:  67     ISSN:  0022-538X     ISO Abbreviation:  J. Virol.     Publication Date:  1993 Jun 
Date Detail:
Created Date:  1993-06-21     Completed Date:  1993-06-21     Revised Date:  2009-11-18    
Medline Journal Info:
Nlm Unique ID:  0113724     Medline TA:  J Virol     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  3507-14     Citation Subset:  IM    
Affiliation:
Department of Molecular Biology, Howard Hughes Medical Institute, Princeton University, New Jersey 08544-1014.
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MeSH Terms
Descriptor/Qualifier:
Adenoviruses, Human / enzymology*,  genetics,  growth & development
Amino Acid Sequence
Endopeptidases / genetics,  metabolism*
Fluorescent Antibody Technique
Hela Cells
Humans
Intermediate Filaments / metabolism*,  ultrastructure
Keratins / isolation & purification,  metabolism*
Molecular Sequence Data
Mutagenesis
Time Factors
Grant Support
ID/Acronym/Agency:
CA 41086/CA/NCI NIH HHS
Chemical
Reg. No./Substance:
68238-35-7/Keratins; EC 3.4.-/Endopeptidases
Comments/Corrections

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