| An adenoviral system for tetracycline-regulated TGF-beta expression mediates a reversible cell cycle arrest. | |
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MedLine Citation:
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PMID: 11767279 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The ability to regulate the proliferation of normal cells in a reversible manner would be a useful adjunct to some clinical therapies. including many types of cancer chemotherapy and surgery for periodontal regeneration. While the application of recombinant growth factors and cytokines to target cells is a logical approach to regulate cell proliferation, the high turnover rates of these peptide factors often make this approach impractical. Recombinant adenoviral vectors can be used to direct the expression of transgene products such as growth factors in many cell types in vitro and in vivo. We have adapted the tetracycline-regulated expression system to allow regulated transforming growth factor (TGF)-beta1 expression using recombinant adenovirus. We demonstrate that infection with a recombinant TGF-beta-encoding virus system in primary human oral keratinocytes and in a lung epithelial cell line is sufficient to allow a cell cycle arrest that is reversible upon tetracycline addition. This inhibition is efficient even after the infection of a minority of cells in a population. These results highlight the possibility of using low level infection with recombinant adenovirus to cause short-term blocks on cell proliferation. |
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Authors:
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M A Wettergreen; J W Hunniford; J M Crawford; G R Adami |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: European journal of oral sciences Volume: 109 ISSN: 0909-8836 ISO Abbreviation: Eur. J. Oral Sci. Publication Date: 2001 Dec |
Date Detail:
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Created Date: 2001-12-19 Completed Date: 2002-01-30 Revised Date: 2008-11-21 |
Medline Journal Info:
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Nlm Unique ID: 9504563 Medline TA: Eur J Oral Sci Country: Denmark |
Other Details:
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Languages: eng Pagination: 415-21 Citation Subset: D; IM |
Affiliation:
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Department of Oral Medicine and Diagnostic Sciences, College of Dentistry, University of Illinois at Chicago, USA. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Adenoviridae
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genetics* Adenovirus E1 Proteins / genetics Cell Cycle / drug effects, physiology* Epithelial Cells / cytology*, drug effects Gene Expression / drug effects* Gene Transfer Techniques Genetic Vectors Humans Promoter Regions, Genetic / drug effects Recombinant Proteins / pharmacology Tetracycline / pharmacology* Trans-Activators / drug effects Transforming Growth Factor beta / biosynthesis*, genetics, physiology Transgenes / physiology |
| Chemical | |
Reg. No./Substance:
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0/Adenovirus E1 Proteins; 0/Recombinant Proteins; 0/Trans-Activators; 0/Transforming Growth Factor beta; 60-54-8/Tetracycline |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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