Document Detail

The adaptor protein p66Shc is a positive regulator in the angiogenic response induced by hypoxic T cells.
MedLine Citation:
PMID:  19889727     Owner:  NLM     Status:  MEDLINE    
Immune cells play an important role in the onset of angiogenesis. Here, we report that VEGF represents the major proangiogenic factor expressed by T cells exposed to hypoxia, a common feature of inflammation and tumor microenvironment. The supernatants of hypoxic T cells were highly angiogenic when delivered on the chick embryo CAM. The angiogenic response was abrogated by a neutralizing anti-VEGF antibody and mimicked by rVEGF. Interestingly, VEGF induction by hypoxia was up-regulated in Jurkat T cells overexpressing the adaptor protein p66Shc but not the inactive S36 p66Shc mutant, and it was abolished in p66Shc-/- mouse splenocytes. Accordingly, the angiogenic response induced by the supernatants from hypoxic p66Shc-/- splenocytes was reduced dramatically when compared with the wild-type controls. In conclusion, hypoxic T cells may contribute to the onset of angiogenesis through a novel VEGF-mediated mechanism, where p66Shc acts as a positive regulator.
Antonella Naldini; Emilia Morena; Annalisa Pucci; Michela Pellegrini; Cosima T Baldari; Pier Giuseppe Pelicci; Marco Presta; Domenico Ribatti; Fabio Carraro
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2009-11-04
Journal Detail:
Title:  Journal of leukocyte biology     Volume:  87     ISSN:  1938-3673     ISO Abbreviation:  J. Leukoc. Biol.     Publication Date:  2010 Mar 
Date Detail:
Created Date:  2010-03-02     Completed Date:  2010-04-02     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  8405628     Medline TA:  J Leukoc Biol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  365-9     Citation Subset:  IM    
Department of Physiology, University of Siena, Via Aldo Moro, 53100 Siena, Italy.
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MeSH Terms
Cell Hypoxia
Chick Embryo
Gene Deletion
Gene Expression Regulation
Hypoxia-Inducible Factor 1, alpha Subunit / metabolism
Jurkat Cells
Leukocytes, Mononuclear / metabolism
Neovascularization, Physiologic*
Shc Signaling Adaptor Proteins / metabolism*
T-Lymphocytes / cytology*,  metabolism*
Vascular Endothelial Growth Factor A / genetics,  metabolism
Reg. No./Substance:
0/Hypoxia-Inducible Factor 1, alpha Subunit; 0/SHC1 protein, human; 0/Shc Signaling Adaptor Proteins; 0/Shc1 protein, mouse; 0/Vascular Endothelial Growth Factor A
Comment In:
J Leukoc Biol. 2010 Mar;87(3):359-61   [PMID:  20194161 ]

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