| The adaptor protein p66Shc is a positive regulator in the angiogenic response induced by hypoxic T cells. | |
| | |
MedLine Citation:
|
PMID: 19889727 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
Immune cells play an important role in the onset of angiogenesis. Here, we report that VEGF represents the major proangiogenic factor expressed by T cells exposed to hypoxia, a common feature of inflammation and tumor microenvironment. The supernatants of hypoxic T cells were highly angiogenic when delivered on the chick embryo CAM. The angiogenic response was abrogated by a neutralizing anti-VEGF antibody and mimicked by rVEGF. Interestingly, VEGF induction by hypoxia was up-regulated in Jurkat T cells overexpressing the adaptor protein p66Shc but not the inactive S36 p66Shc mutant, and it was abolished in p66Shc-/- mouse splenocytes. Accordingly, the angiogenic response induced by the supernatants from hypoxic p66Shc-/- splenocytes was reduced dramatically when compared with the wild-type controls. In conclusion, hypoxic T cells may contribute to the onset of angiogenesis through a novel VEGF-mediated mechanism, where p66Shc acts as a positive regulator. |
| | |
Authors:
|
Antonella Naldini; Emilia Morena; Annalisa Pucci; Michela Pellegrini; Cosima T Baldari; Pier Giuseppe Pelicci; Marco Presta; Domenico Ribatti; Fabio Carraro |
Related Documents
:
|
20970377 - Mixotrophy in ciliates. 15781267 - Suppression of pi3k/mtor pathway rescues llc cells from cell death induced by hypoxia. 18231777 - N-hexane toxicity in jurkat t-cells is mediated by reactive oxygen species. 17550967 - Role of the small gtpase rhoa in the hypoxia-induced decrease of plasma membrane na,k-a... 6321217 - A new system for the study of erythroid cell differentiation. 12445497 - Autocrine/paracrine regulation of apoptosis in epithelial cells by prostaglandin e2. |
Publication Detail:
|
Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2009-11-04 |
Journal Detail:
|
Title: Journal of leukocyte biology Volume: 87 ISSN: 1938-3673 ISO Abbreviation: J. Leukoc. Biol. Publication Date: 2010 Mar |
Date Detail:
|
Created Date: 2010-03-02 Completed Date: 2010-04-02 Revised Date: - |
Medline Journal Info:
|
Nlm Unique ID: 8405628 Medline TA: J Leukoc Biol Country: United States |
Other Details:
|
Languages: eng Pagination: 365-9 Citation Subset: IM |
Affiliation:
|
Department of Physiology, University of Siena, Via Aldo Moro, 53100 Siena, Italy. naldini@unisi.it |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Animals Cell Hypoxia Chick Embryo Gene Deletion Gene Expression Regulation Humans Hypoxia-Inducible Factor 1, alpha Subunit / metabolism Jurkat Cells Leukocytes, Mononuclear / metabolism Mice Neovascularization, Physiologic* Shc Signaling Adaptor Proteins / metabolism* T-Lymphocytes / cytology*, metabolism* Vascular Endothelial Growth Factor A / genetics, metabolism |
| Chemical | |
Reg. No./Substance:
|
0/Hypoxia-Inducible Factor 1, alpha Subunit; 0/SHC1 protein, human; 0/Shc Signaling Adaptor Proteins; 0/Shc1 protein, mouse; 0/Vascular Endothelial Growth Factor A |
| Comments/Corrections | |
Comment In:
|
J Leukoc Biol. 2010 Mar;87(3):359-61
[PMID:
20194161
]
|
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Phagosomal retention of Francisella tularensis results in TIRAP/Mal-independent TLR2 signaling.
Next Document: Adenosine A2A receptor activation limits graft-versus-host disease after allogenic hematopoietic ste...