| The adaptive response of anaerobically grown Saccharomyces cerevisiae to hydrogen peroxide is mediated by the Yap1 and Skn7 transcription factors. | |
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MedLine Citation:
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PMID: 18795957 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The molecular mechanisms involved in the ability of cells to adapt and respond to differing oxygen tensions are of great interest to the pharmaceutical, medical and fermentation industries. The transcriptional profiles reported in previous studies of cells grown under anaerobic, aerobic and dynamic growth conditions have shown significantly altered responses including induction of genes regulated by the oxidative stress transcription factor Yap1p when oxygen was present. The present study investigated the phenotypic changes that occur in cells when shifted from anaerobic to aerobic growth conditions and it was found through mutant analyses that the elevated activity of Yap1p during the shift was mediated by the phospholipid hydroperoxide-sensing protein encoded by GPX3. Cell viability and growth rate were unaffected even though anaerobically grown cells were found to be hypersensitive to low doses of the oxidative stress-inducing compound hydrogen peroxide (H(2)O(2)). Adaptation to H(2)O(2) treatment was demonstrated to occur when anaerobically grown wild-type cells were aerated for a short time that was reliant on the Yap1p and Skn7p transcription factors. |
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Authors:
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Anthony G Beckhouse; Chris M Grant; Peter J Rogers; Ian W Dawes; Vincent J Higgins |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2008-09-15 |
Journal Detail:
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Title: FEMS yeast research Volume: 8 ISSN: 1567-1356 ISO Abbreviation: FEMS Yeast Res. Publication Date: 2008 Dec |
Date Detail:
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Created Date: 2008-12-04 Completed Date: 2009-01-12 Revised Date: 2009-07-24 |
Medline Journal Info:
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Nlm Unique ID: 101085384 Medline TA: FEMS Yeast Res Country: England |
Other Details:
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Languages: eng Pagination: 1214-22 Citation Subset: IM |
Affiliation:
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Ramaciotti Centre for Gene Function Analysis, School of Biotechnology and Biomolecular Sciences, University of New South Wales, Sydney, Australia. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Adaptation, Physiological* Anaerobiosis DNA-Binding Proteins / metabolism* Gene Expression Regulation, Fungal Hydrogen Peroxide / pharmacology* Mutation Oxidative Stress* Saccharomyces cerevisiae / drug effects*, genetics, growth & development* Saccharomyces cerevisiae Proteins / genetics, metabolism* Transcription Factors / metabolism* |
| Grant Support | |
ID/Acronym/Agency:
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//Wellcome Trust |
| Chemical | |
Reg. No./Substance:
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0/DNA-Binding Proteins; 0/SKN7 protein, S cerevisiae; 0/Saccharomyces cerevisiae Proteins; 0/Transcription Factors; 0/YAP1 protein, S cerevisiae; 7722-84-1/Hydrogen Peroxide |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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