| The acute toxic effects of particulate matter in mouse lung are related to size and season of collection. | |
| | |
MedLine Citation:
|
PMID: 21371539 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
The toxicity of size-fractionated particulate matter (PM10 and PM2.5) collected in Milano during two different seasons (summer and winter) has been evaluated in vivo. The focus is on time related (3 h, 24 h and 1 week) lung response following a single intratracheal aerosolization in BALB/c mice. The bronchoalveolar lavage fluid (BALf) and the lung parenchyma were screened for different markers of inflammation and cytotoxicity. Histology and immunohistochemistry were performed on excised fixed lungs to assess the effects produced by the different PM fractions. All the analyzed inflammatory markers (PMNs percentage, TNF-α, Hsp70 in the BALf, HO-1 in lung parenchyma), increased after summer PM10 administration; on the contrary winter PM10 and PM2.5 specifically increased the amount of the Cyp1B1, a protein putatively involved in the induction of pro-carcinogenic effect. Moreover, we detected an intensification of LDH activity in the BALf after the administration of winter PM10 and PM2.5, potentially related to an in progress necrotic process while after summer PM10 and PM2.5 administration, the initiation of the caspase cascade suggested a cytotoxic effect sustained by apoptosis. Our results evidenced the toxicity mechanisms elicited by size fractionated PM samples, collected in winter and summer seasons, which differs for dimensions, chemical and microbiological composition. PM10 has been indicated to elicit above all a pro-inflammatory response, linked to its specific biological components, while PM2.5 is supposed to be more harmful due to its smaller dimension and the ability to distribute into the lung alveolar districts. We hypothesized that adverse health effects observed after a single dose of winter PM2.5 is at least partly caused by specific winter PM components, i.e. PAH and transitional metals. |
| | |
Authors:
|
Francesca Farina; Giulio Sancini; Paride Mantecca; Daniele Gallinotti; Marina Camatini; Paola Palestini |
Related Documents
:
|
21343299 - Keratinocyte growth factor augments pulmonary innate immunity through epithelium-driven... 15100659 - Clinical and laboratory evaluation of complement deficiency. 6233339 - Circulating immune complexes: their immunochemistry, biology, and detection in selected... 10600339 - Immune complex size and complement regulate cytokine production by peripheral blood mon... 2980129 - Function of the follicular dendritic cell in the germinal center of lymphoid follicles. 7081809 - Mechanisms of complement-mediated clearance of bacteria from the murine lung. 15149329 - Gentamicin treatment induces simultaneous mesangial proliferation and apoptosis in rats. 12354279 - Characterization of chemokines and their receptors in the central nervous system: physi... 22153609 - Assessment of clinicopathological characteristics and immunoexpression of cox-2 and il-... |
Publication Detail:
|
Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2011-03-01 |
Journal Detail:
|
Title: Toxicology letters Volume: 202 ISSN: 1879-3169 ISO Abbreviation: Toxicol. Lett. Publication Date: 2011 May |
Date Detail:
|
Created Date: 2011-04-18 Completed Date: 2011-06-14 Revised Date: 2012-01-24 |
Medline Journal Info:
|
Nlm Unique ID: 7709027 Medline TA: Toxicol Lett Country: Netherlands |
Other Details:
|
Languages: eng Pagination: 209-17 Citation Subset: IM |
Copyright Information:
|
Copyright © 2011 Elsevier Ireland Ltd. All rights reserved. |
Affiliation:
|
Department of Experimental Medicine, POLARIS Research Center, University of Milano-Bicocca, 48 via Cadore, Monza 20052, Italy. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Air Pollutants
/
toxicity* Animals Aryl Hydrocarbon Hydroxylases / metabolism Biological Markers / metabolism Bronchoalveolar Lavage Fluid / chemistry, cytology HSP70 Heat-Shock Proteins / metabolism Heme Oxygenase-1 / metabolism Italy Lung / drug effects*, metabolism, pathology Male Mice Mice, Inbred BALB C Particle Size* Particulate Matter / toxicity* Seasons* Toxicity Tests, Acute Tumor Necrosis Factor-alpha / metabolism |
| Chemical | |
Reg. No./Substance:
|
0/Air Pollutants; 0/Biological Markers; 0/HSP70 Heat-Shock Proteins; 0/Particulate Matter; 0/Tumor Necrosis Factor-alpha; EC 1.14.14.1/Aryl Hydrocarbon Hydroxylases; EC 1.14.14.1/cytochrome P450, family 1, subfamily B, polypeptide 1; EC 1.14.99.3/Heme Oxygenase-1 |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Effects of piracetam on behavior and learning in adult zebrafish.
Next Document: Nanosuspension for improving the bioavailability of a poorly soluble drug and screening of stabilizi...