| An acute model for IgA-mediated glomerular inflammation in rats induced by monoclonal polymeric rat IgA antibodies. | |
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MedLine Citation:
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PMID: 8099859 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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An acute model for IgA-mediated glomerular inflammation in rats was induced by the in situ deposition of IgA directly into the glomerular mesangium. F(ab')2 anti-Thy1 MoAb was used to anchor an antigen, DNP (2,4-dinitrophenol), in the glomeruli of rats. Subsequent infusion of rat polymeric (p-) or monomeric (m-) IgA MoAb with specificity for DNP resulted in mesangial deposition of IgA in both groups of rats. However, acute proteinuria was observed only in p-IgA-treated rats and not in PBS- or m-IgA-treated rats. Immunofluorescence analysis revealed deposition of C3 in an identical pattern to that of IgA in the glomeruli of p-IgA-treated rats. No mesangial deposits of C4 or C1q were seen in these animals. Rats receiving m-IgA or PBS displayed no detectable C3, C4 or C1q deposition. The amount of proteinuria in p-IgA-treated rats was related to the amount of deposited C3. The presence of intraglomerular monocytes was only observed 2 days after p-IgA injection. By light microscopy, aneurysm formation, mesangial hypercellularity and matrix expansion were seen only in p-IgA-treated rats. However, by 37 days post-injection complete resolution of the lesions was observed. No histological renal changes were observed in PBS- or m-IgA-treated rats. In conclusion, an acute form of IgA-mediated nephritis in rats was induced by p-IgA but not by m-IgA. This reproducible model provides a basis for further study into the mechanisms of IgA-mediated glomerular inflammation. |
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Authors:
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R K Stad; J A Bruijn; D J van Gijlswijk-Janssen; L A van Es; M R Daha |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Clinical and experimental immunology Volume: 92 ISSN: 0009-9104 ISO Abbreviation: Clin. Exp. Immunol. Publication Date: 1993 Jun |
Date Detail:
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Created Date: 1993-07-20 Completed Date: 1993-07-20 Revised Date: 2009-11-18 |
Medline Journal Info:
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Nlm Unique ID: 0057202 Medline TA: Clin Exp Immunol Country: ENGLAND |
Other Details:
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Languages: eng Pagination: 514-21 Citation Subset: IM |
Affiliation:
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Department of Nephrology, University Hospital Leiden, The Netherlands. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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2,4-Dinitrophenol Acute Disease Animals Antibodies, Monoclonal Antigens, Surface / immunology Antigens, Thy-1 Complement Hemolytic Activity Assay Complement System Proteins / immunology Dinitrophenols / immunology Disease Models, Animal Fluorescent Antibody Technique Glomerular Mesangium / immunology Glomerulonephritis, IGA / immunology*, pathology Hematuria / pathology Immunoglobulin A Male Membrane Glycoproteins / immunology Polymers Proteinuria / pathology Rats Rats, Wistar |
| Chemical | |
Reg. No./Substance:
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0/Antibodies, Monoclonal; 0/Antigens, Surface; 0/Antigens, Thy-1; 0/Dinitrophenols; 0/Immunoglobulin A; 0/Membrane Glycoproteins; 0/Polymers; 51-28-5/2,4-Dinitrophenol; 9007-36-7/Complement System Proteins |
| Comments/Corrections | |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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