| The activity of ornithine transcarbamoylase and arginase during mechanical jaundice in the rat model. | |
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MedLine Citation:
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PMID: 15916970 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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BACKGROUND: The pathogenesis of morbidity and mortality associated with intervention of the biliary system in patients with obstructive jaundice is unknown. Mechanical jaundice initiates the development of morphological changes in hepatocytes with concomitant disturbances in metabolism. These are followed by changes in enzyme activity in hepatocytes and peripheral blood. MATERIAL AND METHODS: Wistar rats were divided into three groups: group 1, sham-operated controls; group 2, rats with permanent jaundice; and group 3, rats with temporary mechanical jaundice. The animals were examined at 2 weeks (groups A), 4 weeks (groups B) and 6 weeks (groups C) after surgery. We explored the impact of induced mechanical jaundice on the activity of selected urea cycle enzymes (arginase [E.C.3.5.3.1] and ornithine transcarbamoylase (OTC) [E.C.2.1.3.3]). RESULTS: Mechanical jaundice was found to induce changes in hepatocytic metabolism, which in turn led to disturbances in the urea cycle and the process of transamination. After relief of the mechanical jaundice (recanalization of the common bile duct), the urea cycle activity in the liver was greatly increased despite the normalization of the basic biochemical indices. CONCLUSION: The results of the experiment confirmed the hypothesis that long-term mechanical jaundice causes lasting disturbances in hepatocytic metabolism. We conclude that the rate of nitrogen metabolism is higher after recanalization of the bile duct. |
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Authors:
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Brygida Beck; Marian Ciszek; Renata Polaniak; Zbigniew Beyga; Wojciech Król; Marek Drozdz; Jashovam Shani |
Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: The Journal of surgical research Volume: 126 ISSN: 0022-4804 ISO Abbreviation: J. Surg. Res. Publication Date: 2005 Jun |
Date Detail:
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Created Date: 2005-05-26 Completed Date: 2005-07-28 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 0376340 Medline TA: J Surg Res Country: United States |
Other Details:
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Languages: eng Pagination: 19-26 Citation Subset: IM |
Affiliation:
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Department of Biochemistry, Medical University of Silesia, Zabrze, Poland. bbeck@slam.katowice.pl |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Alkaline Phosphatase
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blood Animals Arginase / metabolism* Bilirubin / blood Disease Models, Animal Jaundice, Obstructive / blood, enzymology* Male Ornithine Carbamoyltransferase / metabolism* Rats Rats, Wistar Urea / metabolism |
| Chemical | |
Reg. No./Substance:
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57-13-6/Urea; 635-65-4/Bilirubin; EC 2.1.3.3/Ornithine Carbamoyltransferase; EC 3.1.3.1/Alkaline Phosphatase; EC 3.5.3.1/Arginase |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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