Document Detail


The activity and expression of NTPDase is altered in lymphocytes of multiple sclerosis patients.
MedLine Citation:
PMID:  19914228     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
BACKGROUND: Multiple sclerosis (MS) is a demyelinating neurological disease, which is presumed to be a consequence of infiltrating lymphocytes that are autoreactive to myelin proteins. ATP and adenosine contribute to fine-tuning immune responses and NTPDase (CD39) and adenosine deaminase (ADA) are important enzymes in the control of the extracellular levels of these molecules at the site of inflammation. We evaluated the activity and expression of NTPDase and adenosine deaminase (ADA) activity in lymphocytes from patients with the relapsing-remitting form of MS (RRMS). METHODS: This study involved 22 patients with RRMS and 22 healthy subjects as a control group. The lymphocytes were isolated from blood and separated on Ficoll density gradients and after isolation the NTPDase and ADA activities were determined. RESULTS: The NTPDase activity and expression were increased in lymphocytes from RRMS patients when compared with the control group (p<0.05). In addition, a decrease in ADA activity was observed in lymphocytes from these patients when compared to the control group (p<0.05). CONCLUSIONS: The regulation of ATP and adenosine levels by NTPDase and ADA activities may be important to preserve cellular integrity and to modulate the immune response in MS.
Authors:
Roselia M Spanevello; Cinthia M Mazzanti; Roberta Schmatz; Gustavo Thom?; Margarete Bagatini; Maisa Correa; Cintia Rosa; Naiara Stefanello; Luziane Potrich Bell?; Maria B Moretto; Liliane Oliveira; Vera M Morsch; Maria R C Schetinger
Related Documents :
21543778 - Laboratory variables associated with low near-infrared cerebral oxygen saturation in ic...
6472538 - Immunologic abnormalities in hemodialysis patients: improvement after pyridoxine therapy.
11268478 - Detection of circulating malignant cells by rt-pcr in long-term clinically disease-free...
1770368 - Preoperative nutritional status of total joint patients. relationship to postoperative ...
23896488 - Airway evaluation by indirect laryngoscopy in patients with lingual tonsillar hypertrophy.
8266158 - Spontaneous pneumothorax: a review of 29 admissions into hospital universiti sains mala...
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2009-11-13
Journal Detail:
Title:  Clinica chimica acta; international journal of clinical chemistry     Volume:  411     ISSN:  1873-3492     ISO Abbreviation:  Clin. Chim. Acta     Publication Date:  2010 Feb 
Date Detail:
Created Date:  2010-01-27     Completed Date:  2010-03-25     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  1302422     Medline TA:  Clin Chim Acta     Country:  Netherlands    
Other Details:
Languages:  eng     Pagination:  210-4     Citation Subset:  IM    
Copyright Information:
Copyright 2009 Elsevier B.V. All rights reserved.
Affiliation:
Departamento de Bioqu?mica, Instituto de Ci?ncias B?sicas da Sa?de, Universidade Federal do Rio Grande do Sul, Rua Ramiro Barcellos, 2600-Anexo, 90035-003 Porto Alegre, RS, Brazil.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Descriptor/Qualifier:
Adenosine Deaminase / metabolism
Adenosine Diphosphate / metabolism
Adenosine Triphosphate / metabolism
Adult
Aged
Antigens, CD / metabolism*
Apyrase / metabolism*
Case-Control Studies
Female
Gene Expression Regulation, Enzymologic*
Humans
Lymphocytes / metabolism*
Male
Middle Aged
Multiple Sclerosis / enzymology*,  immunology*,  metabolism
Recurrence
Chemical
Reg. No./Substance:
0/Antigens, CD; 56-65-5/Adenosine Triphosphate; 58-64-0/Adenosine Diphosphate; EC 3.5.4.4/Adenosine Deaminase; EC 3.6.1.5/Apyrase; EC 3.6.1.5/CD39 antigen

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


Previous Document:  Ensuring comparability of benzene exposure estimates across three nested case-control studies in the...
Next Document:  The clinical implications of MMP-11 and CK-20 expression in human breast cancer.