| The activity and expression of NTPDase is altered in lymphocytes of multiple sclerosis patients. | |
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MedLine Citation:
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PMID: 19914228 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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BACKGROUND: Multiple sclerosis (MS) is a demyelinating neurological disease, which is presumed to be a consequence of infiltrating lymphocytes that are autoreactive to myelin proteins. ATP and adenosine contribute to fine-tuning immune responses and NTPDase (CD39) and adenosine deaminase (ADA) are important enzymes in the control of the extracellular levels of these molecules at the site of inflammation. We evaluated the activity and expression of NTPDase and adenosine deaminase (ADA) activity in lymphocytes from patients with the relapsing-remitting form of MS (RRMS). METHODS: This study involved 22 patients with RRMS and 22 healthy subjects as a control group. The lymphocytes were isolated from blood and separated on Ficoll density gradients and after isolation the NTPDase and ADA activities were determined. RESULTS: The NTPDase activity and expression were increased in lymphocytes from RRMS patients when compared with the control group (p<0.05). In addition, a decrease in ADA activity was observed in lymphocytes from these patients when compared to the control group (p<0.05). CONCLUSIONS: The regulation of ATP and adenosine levels by NTPDase and ADA activities may be important to preserve cellular integrity and to modulate the immune response in MS. |
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Authors:
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Roselia M Spanevello; Cinthia M Mazzanti; Roberta Schmatz; Gustavo Thom?; Margarete Bagatini; Maisa Correa; Cintia Rosa; Naiara Stefanello; Luziane Potrich Bell?; Maria B Moretto; Liliane Oliveira; Vera M Morsch; Maria R C Schetinger |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2009-11-13 |
Journal Detail:
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Title: Clinica chimica acta; international journal of clinical chemistry Volume: 411 ISSN: 1873-3492 ISO Abbreviation: Clin. Chim. Acta Publication Date: 2010 Feb |
Date Detail:
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Created Date: 2010-01-27 Completed Date: 2010-03-25 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 1302422 Medline TA: Clin Chim Acta Country: Netherlands |
Other Details:
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Languages: eng Pagination: 210-4 Citation Subset: IM |
Copyright Information:
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Copyright 2009 Elsevier B.V. All rights reserved. |
Affiliation:
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Departamento de Bioqu?mica, Instituto de Ci?ncias B?sicas da Sa?de, Universidade Federal do Rio Grande do Sul, Rua Ramiro Barcellos, 2600-Anexo, 90035-003 Porto Alegre, RS, Brazil. |
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| MeSH Terms | |
Descriptor/Qualifier:
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Adenosine Deaminase
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metabolism Adenosine Diphosphate / metabolism Adenosine Triphosphate / metabolism Adult Aged Antigens, CD / metabolism* Apyrase / metabolism* Case-Control Studies Female Gene Expression Regulation, Enzymologic* Humans Lymphocytes / metabolism* Male Middle Aged Multiple Sclerosis / enzymology*, immunology*, metabolism Recurrence |
| Chemical | |
Reg. No./Substance:
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0/Antigens, CD; 56-65-5/Adenosine Triphosphate; 58-64-0/Adenosine Diphosphate; EC 3.5.4.4/Adenosine Deaminase; EC 3.6.1.5/Apyrase; EC 3.6.1.5/CD39 antigen |
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