| An active product of cruciferous vegetables, 3,3'-diindolylmethane, inhibits invasive properties of extravillous cytotrophoblastic cells. | |
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MedLine Citation:
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PMID: 22592193 Owner: NLM Status: Publisher |
Abstract/OtherAbstract:
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OBJECTIVES: During implantation, human trophoblastic cells have to proliferate, migrate and invade pregnant uterus. A natural product of cruciferous vegetables, 3,3'-diindolylmethane (DIM), is known to induce some stress response genes (such as glucose-regulated protein 78 kDa (GRP78)) and to have anti-invasive and pro-apoptotic effects on tumor cells. Therefore, we have investigated the potential effect of DIM on invasive extravillous cytotrophoblasts (evCTBs) cells. Materials and Methods: evCTBs were purified from first trimester trophoblasts and cultured in presence or not of DIM for 48h. In order to evaluate invasive properties of cells, they were seeded on collagen-coated insert following boyden chamber principle and matrix metalloproteinases (MMPs) and GRP78 expression was evaluated by qPCR. Results: We showed that DIM decreases (p=0.013) invasive properties of evCTBs. In parallel, we determined that MMP-2, -7 and -9 which are involved in evCTBs invasion and known to be regulated by DIM, are not affected by DIM in evCTBs. In contrast, MMP-1 mRNA is induced (p=0.03) and MMP-12 is decreased (p=0.01) in DIM treated cells. Moreover, DIM treatment does not affect GRP78 mRNA expression in evCTBs. Conclusions: Collectively, the present results provide evidence that DIM does not impact evenly on evCTBs and cancer cells. |
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Authors:
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Pascale Ribaux; Olivier Irion; Marie Cohen |
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Publication Detail:
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Type: JOURNAL ARTICLE Date: 2012-4-17 |
Journal Detail:
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Title: Neuro endocrinology letters Volume: 33 ISSN: 0172-780X ISO Abbreviation: - Publication Date: 2012 Apr |
Date Detail:
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Created Date: 2012-5-17 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 8008373 Medline TA: Neuro Endocrinol Lett Country: - |
Other Details:
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Languages: ENG Pagination: 133-137 Citation Subset: - |
Affiliation:
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Laboratoire d'Hormonologie, Department of Gynaecology and Obstetrics, HUG, Maternité, Switzerland. marie.cohen@hcuge.ch. |
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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