| An active enzyme constructed from a 9-amino acid alphabet. | |
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MedLine Citation:
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PMID: 16144843 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Nature employs a set of 20 amino acids to produce a repertoire of protein structures endowed with sophisticated functions. Here, we combined design and selection to create an enzyme composed entirely from a set of only 9 amino acids that can rescue auxotrophic cells lacking chorismate mutase. The simplified protein captures key structural features of its natural counterpart but appears to be somewhat less stable and more flexible. The potential of a dramatically reduced amino acid alphabet to produce an active catalyst supports the notion that primordial enzymes may have possessed low amino acid diversity and suggests that combinatorial engineering strategies, such as the one used here, may be generally applied to create enzymes with novel structures and functions. |
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Authors:
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Kai U Walter; Katherina Vamvaca; Donald Hilvert |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2005-09-06 |
Journal Detail:
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Title: The Journal of biological chemistry Volume: 280 ISSN: 0021-9258 ISO Abbreviation: J. Biol. Chem. Publication Date: 2005 Nov |
Date Detail:
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Created Date: 2005-11-07 Completed Date: 2006-01-12 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 2985121R Medline TA: J Biol Chem Country: United States |
Other Details:
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Languages: eng Pagination: 37742-6 Citation Subset: IM |
Affiliation:
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Laboratory of Organic Chemistry, Swiss Federal Institute of Technology, ETH Hönggerberg, Zürich. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Amino Acid Sequence Amino Acids / analysis*, chemistry Chorismate Mutase / chemistry*, metabolism* Enzyme Stability Evolution, Molecular Models, Molecular Molecular Sequence Data Pliability Protein Conformation Protein Engineering / methods* Sequence Homology, Amino Acid Temperature |
| Chemical | |
Reg. No./Substance:
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0/Amino Acids; EC 5.4.99.5/Chorismate Mutase |
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