Document Detail


An active enzyme constructed from a 9-amino acid alphabet.
MedLine Citation:
PMID:  16144843     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Nature employs a set of 20 amino acids to produce a repertoire of protein structures endowed with sophisticated functions. Here, we combined design and selection to create an enzyme composed entirely from a set of only 9 amino acids that can rescue auxotrophic cells lacking chorismate mutase. The simplified protein captures key structural features of its natural counterpart but appears to be somewhat less stable and more flexible. The potential of a dramatically reduced amino acid alphabet to produce an active catalyst supports the notion that primordial enzymes may have possessed low amino acid diversity and suggests that combinatorial engineering strategies, such as the one used here, may be generally applied to create enzymes with novel structures and functions.
Authors:
Kai U Walter; Katherina Vamvaca; Donald Hilvert
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2005-09-06
Journal Detail:
Title:  The Journal of biological chemistry     Volume:  280     ISSN:  0021-9258     ISO Abbreviation:  J. Biol. Chem.     Publication Date:  2005 Nov 
Date Detail:
Created Date:  2005-11-07     Completed Date:  2006-01-12     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  2985121R     Medline TA:  J Biol Chem     Country:  United States    
Other Details:
Languages:  eng     Pagination:  37742-6     Citation Subset:  IM    
Affiliation:
Laboratory of Organic Chemistry, Swiss Federal Institute of Technology, ETH Hönggerberg, Zürich.
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MeSH Terms
Descriptor/Qualifier:
Amino Acid Sequence
Amino Acids / analysis*,  chemistry
Chorismate Mutase / chemistry*,  metabolism*
Enzyme Stability
Evolution, Molecular
Models, Molecular
Molecular Sequence Data
Pliability
Protein Conformation
Protein Engineering / methods*
Sequence Homology, Amino Acid
Temperature
Chemical
Reg. No./Substance:
0/Amino Acids; EC 5.4.99.5/Chorismate Mutase

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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