Document Detail


The activation of membrane targeted CaMK-II in the zebrafish Kupffer's vesicle is required for left-right asymmetry.
MedLine Citation:
PMID:  20630945     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Intracellular calcium ion (Ca(2+)) elevation on the left side of the mouse embryonic node or zebrafish Kupffer's vesicle (KV) is the earliest asymmetric molecular event that is functionally linked to lateral organ placement in these species. In this study, Ca(2+)/CaM-dependent protein kinase (CaMK-II) is identified as a necessary target of this Ca(2+) elevation in zebrafish embryos. CaMK-II is transiently activated in approximately four interconnected cells along the anterior left wall of the KV between the six- and 12-somite stages, which is coincident with known left-sided Ca(2+) elevations. Within these cells, activated CaMK-II is observed at the surface and in clusters, which appear at the base of some KV cilia. Although seven genes encode catalytically active CaMK-II in early zebrafish embryos, one of these genes also encodes a truncated inactive variant (alphaKAP) that can hetero-oligomerize with and target active enzyme to membranes. alphaKAP, beta2 CaMK-II and gamma1 CaMK-II antisense morpholino oligonucleotides, as well as KV-targeted dominant negative CaMK-II, randomize organ laterality and southpaw (spaw) expression in lateral plate mesoderm (LPM). Left-sided CaMK-II activation was most dependent on an intact KV, the PKD2 Ca(2+) channel and gamma1 CaMK-II; however, alphaKAP, beta2 CaMK-II and the RyR3 ryanodine receptor were also necessary for full CaMK-II activation. This is the first report to identify a direct Ca(2+)-sensitive target in left-right asymmetry and supports a model in which membrane targeted CaMK-II hetero-oligomers in nodal cells transduce the left-sided PKD2-dependent Ca(2+) signals to the LPM.
Authors:
Ludmila Francescatto; Sarah C Rothschild; Alexandra L Myers; Robert M Tombes
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Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, Non-P.H.S.     Date:  2010-07-14
Journal Detail:
Title:  Development (Cambridge, England)     Volume:  137     ISSN:  1477-9129     ISO Abbreviation:  Development     Publication Date:  2010 Aug 
Date Detail:
Created Date:  2010-07-28     Completed Date:  2010-08-20     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  8701744     Medline TA:  Development     Country:  England    
Other Details:
Languages:  eng     Pagination:  2753-62     Citation Subset:  IM    
Affiliation:
Department of Biology, Virginia Commonwealth University, Richmond, VA 23284-2012, USA.
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MeSH Terms
Descriptor/Qualifier:
Alternative Splicing
Amino Acid Sequence
Animals
Body Patterning*
Calcium-Calmodulin-Dependent Protein Kinase Type 2 / chemistry,  genetics,  metabolism*
Enzyme Activation
Epithelium / enzymology
Gene Expression Regulation, Developmental
Gene Expression Regulation, Enzymologic
Molecular Sequence Data
Sequence Alignment
Somites / enzymology
Zebrafish / embryology*,  metabolism*
Chemical
Reg. No./Substance:
EC 2.7.11.17/Calcium-Calmodulin-Dependent Protein Kinase Type 2

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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