| The activation of membrane targeted CaMK-II in the zebrafish Kupffer's vesicle is required for left-right asymmetry. | |
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MedLine Citation:
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PMID: 20630945 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Intracellular calcium ion (Ca(2+)) elevation on the left side of the mouse embryonic node or zebrafish Kupffer's vesicle (KV) is the earliest asymmetric molecular event that is functionally linked to lateral organ placement in these species. In this study, Ca(2+)/CaM-dependent protein kinase (CaMK-II) is identified as a necessary target of this Ca(2+) elevation in zebrafish embryos. CaMK-II is transiently activated in approximately four interconnected cells along the anterior left wall of the KV between the six- and 12-somite stages, which is coincident with known left-sided Ca(2+) elevations. Within these cells, activated CaMK-II is observed at the surface and in clusters, which appear at the base of some KV cilia. Although seven genes encode catalytically active CaMK-II in early zebrafish embryos, one of these genes also encodes a truncated inactive variant (alphaKAP) that can hetero-oligomerize with and target active enzyme to membranes. alphaKAP, beta2 CaMK-II and gamma1 CaMK-II antisense morpholino oligonucleotides, as well as KV-targeted dominant negative CaMK-II, randomize organ laterality and southpaw (spaw) expression in lateral plate mesoderm (LPM). Left-sided CaMK-II activation was most dependent on an intact KV, the PKD2 Ca(2+) channel and gamma1 CaMK-II; however, alphaKAP, beta2 CaMK-II and the RyR3 ryanodine receptor were also necessary for full CaMK-II activation. This is the first report to identify a direct Ca(2+)-sensitive target in left-right asymmetry and supports a model in which membrane targeted CaMK-II hetero-oligomers in nodal cells transduce the left-sided PKD2-dependent Ca(2+) signals to the LPM. |
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Authors:
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Ludmila Francescatto; Sarah C Rothschild; Alexandra L Myers; Robert M Tombes |
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Publication Detail:
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Type: Journal Article; Research Support, U.S. Gov't, Non-P.H.S. Date: 2010-07-14 |
Journal Detail:
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Title: Development (Cambridge, England) Volume: 137 ISSN: 1477-9129 ISO Abbreviation: Development Publication Date: 2010 Aug |
Date Detail:
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Created Date: 2010-07-28 Completed Date: 2010-08-20 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 8701744 Medline TA: Development Country: England |
Other Details:
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Languages: eng Pagination: 2753-62 Citation Subset: IM |
Affiliation:
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Department of Biology, Virginia Commonwealth University, Richmond, VA 23284-2012, USA. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Alternative Splicing Amino Acid Sequence Animals Body Patterning* Calcium-Calmodulin-Dependent Protein Kinase Type 2 / chemistry, genetics, metabolism* Enzyme Activation Epithelium / enzymology Gene Expression Regulation, Developmental Gene Expression Regulation, Enzymologic Molecular Sequence Data Sequence Alignment Somites / enzymology Zebrafish / embryology*, metabolism* |
| Chemical | |
Reg. No./Substance:
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EC 2.7.11.17/Calcium-Calmodulin-Dependent Protein Kinase Type 2 |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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