Document Detail

The activated partial thromboplastin time of diluted plasma: variability due to the method of fibrin detection.
MedLine Citation:
PMID:  6722646     Owner:  NLM     Status:  MEDLINE    
The purpose of this study was to determine the effects of the dilution of plasma (1/3 in saline) on the kinetics of fibrin generation in the activated partial thromboplastin time (APTT) assay. The diluted APTT is considered to increase the sensitivity of the APTT test however, studies in our laboratory using an electro-optical fibrin detection system failed to show significant differences in APTT values obtained with diluted and undiluted canine plasma. Seventeen plasmas, including plasmas moderately and markedly deficient in intrinsic factor activity were assayed in the undiluted and diluted APTT assay using two methods for fibrin endpoint detection; a visual "tilt-tube" technique and an electro-optical detection system. In the former technique the endpoint was the formation of a visible fibrin web or clot; in the latter procedure the end point was the first detection of a change in optical density of the plasma. Optical density changes during fibrin formation were also recorded ( thrombokinetograms ). The results indicated that the electro-optical fibrin detection system failed to identify a prolongation of the APTT as a result of 1/3 plasma dilution; a prolongation that was consistently observed with the visual fibrin detection technique. Plasma dilution however, did significantly reduce the rate of fibrin production as indicated by the thrombokinetogram profile. It was concluded that the dilution of plasma with saline, as has been used to increase the sensitivity of the APTT assay procedure, has little effect on the time of onset of fibrin formation in a given plasma. The major effect appears to be on the way in which fibrin forms in that the polymerization/crosslinkage events associated with macroscopic fibrin production are delayed.
I B Johnstone
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Publication Detail:
Type:  Comparative Study; Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Canadian journal of comparative medicine. Revue canadienne de médecine comparée     Volume:  48     ISSN:  0008-4050     ISO Abbreviation:  Can. J. Comp. Med.     Publication Date:  1984 Apr 
Date Detail:
Created Date:  1984-07-23     Completed Date:  1984-07-23     Revised Date:  2009-11-18    
Medline Journal Info:
Nlm Unique ID:  0151747     Medline TA:  Can J Comp Med     Country:  CANADA    
Other Details:
Languages:  eng     Pagination:  198-201     Citation Subset:  IM    
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MeSH Terms
Blood Coagulation Tests / veterinary*
Dog Diseases / blood
Fibrin / biosynthesis*
Hemophilia A / blood,  veterinary
Intrinsic Factor / deficiency
Partial Thromboplastin Time / methods,  veterinary*
Plasma / metabolism*
Sodium Chloride / pharmacology
Reg. No./Substance:
7647-14-5/Sodium Chloride; 9001-31-4/Fibrin; 9008-12-2/Intrinsic Factor

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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