| An activated S6 kinase in regenerating rat liver. | |
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MedLine Citation:
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PMID: 3606618 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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S6 kinase activity was increased in the regenerating liver 5 h after partial hepatectomy compared with sham-operated liver. The protein kinase activity was eluted from DE-52 column at approximately 250 mM NaCl and was not affected by known regulators of protein kinases. The S6 kinase was further purified by chromatography on peptide R1A13-Sepharose 4B and Sephadex G-150. The molecular weight of the enzyme was estimated to be 4.5 X 10(4) by gel filtration. The enzyme catalyzes the phosphorylation of whole histone, mainly H2B histone, at 75 mM Mg2+. These properties are similar to those of a proteolytically modified Ca2+/phospholipid-independent form of protein kinase C. |
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Authors:
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K Mizuta; E Hashimoto; Y Sakanoue; S Nakamura; H Kondo; H Yamamura |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Biochemical and biophysical research communications Volume: 146 ISSN: 0006-291X ISO Abbreviation: Biochem. Biophys. Res. Commun. Publication Date: 1987 Jul |
Date Detail:
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Created Date: 1987-08-27 Completed Date: 1987-08-27 Revised Date: 2009-11-19 |
Medline Journal Info:
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Nlm Unique ID: 0372516 Medline TA: Biochem Biophys Res Commun Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 239-46 Citation Subset: IM |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Amino Acid Sequence Animals Chromatography, Gel Enzyme Activation Histones / metabolism Liver / enzymology* Liver Regeneration* Magnesium / metabolism Phosphorylation Protein Kinase C / metabolism Protein Kinases / metabolism* Rats Ribosomal Protein S6 Kinases Substrate Specificity Time Factors |
| Chemical | |
Reg. No./Substance:
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0/Histones; 7439-95-4/Magnesium; EC 2.7.-/Protein Kinases; EC 2.7.11.1/Ribosomal Protein S6 Kinases; EC 2.7.11.13/Protein Kinase C |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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