| The action of small GTPases rab11 and rab25 in vesicle trafficking during cell migration. | |
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MedLine Citation:
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PMID: 22613965 Owner: NLM Status: In-Data-Review |
Abstract/OtherAbstract:
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Background: The closely related GTPases Rab11 and Rab25 promote cell migration by regulating vesicular transport and recycling of surface receptors. Rab25 carries a constitutively activating mutation in its GTPase domain. Increased expression of Rab25 has been associated with the aggressiveness of migrating tumor cells. Here, we aimed to elucidate potential differences in the role of those two GTPases in vesicle trafficking during cell migration. Methods: We expressed Rab11 and Rab25 wildtype and mutant constructs in HeLa and MDA-MB231 cells and measured their effect on cell morphology, vesicle dynamics and migration behaviour. In prostate cancer samples we analyzed the expression of both GTPases. Results: Cells grown on fibronectin displayed a more stretched morphology when Rab11 was inactivated, whereas inactivation of Rab25 led to reduced stretching. Overexpression of both Rab11 and Rab25 accelerated cell migration. Analysis of vesicular movement revealed higher transport efficiency in the inner cell compartment for Rab11 positive vesicles and in proximity to the membrane for Rab25 positive vesicles. Interestingly, we found Rab25 to be highly expressed in prostate cancer tissue. Conclusion: Taken together, our data suggest that Rab11 is mainly responsible for basal long-distance transport from the rear end to the front of the migrating cell, whereas Rab25 acts predominantly in the small-scale fast recycling within the tips of the cell. Our results further support the idea of Rab25 as a promoter of tumor development. |
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Authors:
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Daniel Kessler; Gianna-Carina Gruen; Dominik Heider; Jessica Morgner; Henning Reis; Schmid Kurt Werner; Verena Jendrossek |
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Publication Detail:
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Type: Journal Article Date: 2012-05-11 |
Journal Detail:
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Title: Cellular physiology and biochemistry : international journal of experimental cellular physiology, biochemistry, and pharmacology Volume: 29 ISSN: 1421-9778 ISO Abbreviation: Cell. Physiol. Biochem. Publication Date: 2012 |
Date Detail:
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Created Date: 2012-05-22 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 9113221 Medline TA: Cell Physiol Biochem Country: Switzerland |
Other Details:
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Languages: eng Pagination: 647-56 Citation Subset: IM |
Copyright Information:
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Copyright © 2012 S. Karger AG, Basel. |
Affiliation:
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Institute of Cell Biology (Cancer Research), Medical School, University of Duisburg - Essen, Essen, Germany. |
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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