| The actin-related protein hArp8 accumulates on the mitotic chromosomes and functions in chromosome alignment. | |
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MedLine Citation:
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PMID: 18163988 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The actin family consists of conventional actin and various actin-related proteins (Arps). Some of these Arps are localized in the nucleus, and a fraction of each of these nuclear Arps is functionally involved in chromatin remodeling and histone acetyltransferase complexes. On the other hand, in mitotic cells, the localization and function of the nuclear Arps are largely unknown. Human Arp8 (hArp8), an ortholog of yeast nuclear Arp8, was recently found to be associated with the hINO80-chromatin remodeling complex along with hArp5. Here we report that hArp8, but not hArp5, accumulates on mitotic chromosomes. This is the first example where a member of the actin family is found to be associated with mitotic chromosomes. Expression of truncated hArp8 proteins and depletion of endogenous hArp8 by RNA interference caused misalignment of mitotic chromosomes, suggesting that chromosome-associated hArp8 has a role in chromosome behavior. In contrast, depletion of hIno80 and hArp5 did not cause misalignment of chromosomes, suggesting that the role of hArp8 at mitotic chromosomes is independent of the activity of hINO80 complexes. These findings provide the first insight into a novel function of actin family members in mitosis. |
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Authors:
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Naoki Aoyama; Asako Oka; Kumiko Kitayama; Hitoshi Kurumizaka; Masahiko Harata |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2007-12-04 |
Journal Detail:
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Title: Experimental cell research Volume: 314 ISSN: 0014-4827 ISO Abbreviation: Exp. Cell Res. Publication Date: 2008 Feb |
Date Detail:
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Created Date: 2008-02-11 Completed Date: 2008-04-07 Revised Date: 2011-10-24 |
Medline Journal Info:
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Nlm Unique ID: 0373226 Medline TA: Exp Cell Res Country: United States |
Other Details:
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Languages: eng Pagination: 859-68 Citation Subset: IM |
Affiliation:
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Laboratory of Molecular Biology, Graduate School of Agricultural Science, Tohoku University, Tsutsumidori-Amamiyamachi 1-1, Aoba-ku, Sendai 981-8555, Japan. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Chromosomes, Mammalian
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chemistry,
physiology* DNA Helicases / physiology Hela Cells Humans Interphase Microfilament Proteins / analysis, chemistry, physiology* Mitosis / genetics* Protein Structure, Tertiary RNA Interference |
| Chemical | |
Reg. No./Substance:
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0/ACTR8 protein, human; 0/Microfilament Proteins; EC 3.6.1.-/DNA Helicases; EC 3.6.1.-/INO80 protein, human |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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