Document Detail


The actin cytoskeleton inhibits pore expansion during PIV5 fusion protein-promoted cell-cell fusion.
MedLine Citation:
PMID:  20537366     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Paramyxovirus fusion (F) proteins promote both virus-cell fusion, required for viral entry, and cell-cell fusion, resulting in syncytia formation. We used the F-actin stabilizing drug, jasplakinolide, and the G-actin sequestrant, latrunculin A, to examine the role of actin dynamics in cell-cell fusion mediated by the parainfluenza virus 5 (PIV5) F protein. Jasplakinolide treatment caused a dose-dependent increase in cell-cell fusion as measured by both syncytia and reporter gene assays, and latrunculin A treatment also resulted in fusion stimulation. Treatment with jasplakinolide or latrunculin A partially rescued a fusion pore opening defect caused by deletion of the PIV5 F protein cytoplasmic tail, but these drugs had no effect on fusion inhibited at earlier stages by either temperature arrest or by a PIV5 heptad repeat peptide. These data suggest that the cortical actin cytoskeleton is an important regulator of fusion pore enlargement, an energetically costly stage of viral fusion protein-mediated membrane merger.
Authors:
Mark A Wurth; Rachel M Schowalter; Everett Clinton Smith; Carole L Moncman; Rebecca Ellis Dutch; Richard O McCann
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Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural    
Journal Detail:
Title:  Virology     Volume:  404     ISSN:  1096-0341     ISO Abbreviation:  Virology     Publication Date:  2010 Aug 
Date Detail:
Created Date:  2010-06-14     Completed Date:  2010-06-29     Revised Date:  2013-05-29    
Medline Journal Info:
Nlm Unique ID:  0110674     Medline TA:  Virology     Country:  United States    
Other Details:
Languages:  eng     Pagination:  117-26     Citation Subset:  IM    
Copyright Information:
Copyright 2010 Elsevier Inc. All rights reserved.
Affiliation:
Department of Molecular and Cellular Biochemistry, University of Kentucky, Lexington, KY 40536-0509, USA.
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MeSH Terms
Descriptor/Qualifier:
Actins / metabolism*
Animals
Bicyclo Compounds, Heterocyclic / metabolism
Cell Fusion*
Cell Line
Cercopithecus aethiops
Cricetinae
Cytoskeleton / metabolism*
Depsipeptides / metabolism
Humans
Models, Biological
Paramyxoviridae / physiology*
Thiazolidines / metabolism
Viral Fusion Proteins / metabolism*
Grant Support
ID/Acronym/Agency:
P20RR20171/RR/NCRR NIH HHS; R01 AI051517-06A2/AI/NIAID NIH HHS; R01 AI051517-08/AI/NIAID NIH HHS; R01A151517//PHS HHS; R21 YE018112//PHS HHS
Chemical
Reg. No./Substance:
0/Actins; 0/Bicyclo Compounds, Heterocyclic; 0/Depsipeptides; 0/Thiazolidines; 0/Viral Fusion Proteins; 102396-24-7/jasplakinolide; 76343-93-6/latrunculin A
Comments/Corrections

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