Document Detail


An acrosomal antigen of human spermatozoa and spermatogenic cells characterized with a monoclonal antibody.
MedLine Citation:
PMID:  6392118     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Monoclonal antibodies were raised against acrosomal antigens of human sperm by immunizing BALB/CA mice with purified ejaculated human spermatozoa. An ELISA-assay, employing glutaraldehyde-fixed spermatozoa as antigen, was used to screen the hybridomas producing anti-human sperm antibodies. Two hybridoma cell-lines produced antibodies which bound to the acrosomal region of spermatozoa. Both gave identical results in preliminary tests and therefore only one was chosen for further experiments. This antibody stained the acrosomal region of fixed but not living spermatozoa by indirect immunofluorescence, indicating an intra-acrosomal localization of the antigen. In acetone-fixed frozen sections of human testis this antigen was expressed only in germ cells in the adluminal compartment of seminiferous tubules. The antigen was clearly visible in round spermatids from the beginning of the cap phase of acrosome development and was also present in premature germ cells which were present in ejaculates and which were in the early stages of acrosome development. By immunochemical analysis this antibody recognized a molecule of 50 K MW as well as other components of 24 to 34 K. The pattern of staining for the antigen was similar in the presence or absence of beta-mercaptoethanol in the sample buffer. The species specificity of the antigen was studied by indirect immunofluorescence using acetone-fixed spermatozoa and the antigen was found to be present in mouse, bovine, ram and boar spermatozoa. This antibody may be useful as an acrosomal marker.
Authors:
M Kallajoki; J Suominen
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  International journal of andrology     Volume:  7     ISSN:  0105-6263     ISO Abbreviation:  Int. J. Androl.     Publication Date:  1984 Aug 
Date Detail:
Created Date:  1985-02-05     Completed Date:  1985-02-05     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  8000141     Medline TA:  Int J Androl     Country:  DENMARK    
Other Details:
Languages:  eng     Pagination:  283-96     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Acrosome / immunology*
Animals
Antibodies, Monoclonal / immunology*
Antigens / analysis,  immunology*
Female
Fluorescent Antibody Technique
Histocytochemistry
Humans
Hybridomas / immunology
Immunoenzyme Techniques
Male
Mice
Mice, Inbred BALB C
Species Specificity
Spermatogenesis
Spermatozoa / immunology*
Testis / immunology
Chemical
Reg. No./Substance:
0/Antibodies, Monoclonal; 0/Antigens

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