| An acid-labile block copolymer of PDMAEMA and PEG as potential carrier for intelligent gene delivery systems. | |
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MedLine Citation:
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PMID: 18088093 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Intelligent gene delivery systems based on physiologically triggered reversible shielding technology have evinced enormous interest due to their potential in vivo applications. In the present work, an acid-labile block copolymer consisting of poly(ethylene glycol) and poly(2-(dimethylamino)ethyl methacrylate) segments connected through a cyclic ortho ester linkage (PEG- a-PDMAEMA) was synthesized by atom transfer radical polymerization of DMAEMA using a PEG macroinitiator with an acid-cleavable end group. PEG- a-PDMAEMA condensed with plasmid DNA formed polyplex nanoparticles with an acid-triggered reversible PEG shield. The pH-dependent shielding/deshielding effect of PEG chains on the polyplex particles were evaluated by zeta potential and size measurements. At pH 7.4, polyplexes generated from PEG- a-PDMAEMA exhibited smaller particle size, lower surface charge, reduced interaction with erythrocytes, and less cytotoxicity compared to PDMAEMA-derived polyplexes. At pH 5.0, zeta potential of polyplexes formed from PEG- a-PDMAEMA increased, leveled up after 2 h of incubation and gradual aggregation occurred in the presence of bovine serum albumin (BSA). In contrast, the stably shielded polyplexes formed by DNA and an acid-stable block copolymer, PEG- b-PDMAEMA, did not change in size and zeta potential in 6 h. In vitro transfection efficiency of the acid-labile copolymer greatly increased after 6 h incubation at pH 5.0, approaching the same level of PDMAEMA, whereas there was only slight increase in efficiency for the stable copolymer, PEG- b-PDMAEMA. |
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Authors:
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Song Lin; Fusheng Du; Yang Wang; Shouping Ji; Dehai Liang; Lei Yu; Zichen Li |
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Publication Detail:
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Type: In Vitro; Journal Article; Research Support, Non-U.S. Gov't Date: 2007-12-19 |
Journal Detail:
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Title: Biomacromolecules Volume: 9 ISSN: 1526-4602 ISO Abbreviation: Biomacromolecules Publication Date: 2008 Jan |
Date Detail:
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Created Date: 2008-01-14 Completed Date: 2008-03-31 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 100892849 Medline TA: Biomacromolecules Country: United States |
Other Details:
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Languages: eng Pagination: 109-15 Citation Subset: IM |
Affiliation:
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Beijing National Laboratory for Molecular Sciences, Key Laboratory of Polymer Chemistry and Physics of Ministry of Education, College of Chemistry & Molecular Engineering, Peking University, Beijing 100871, PR China. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Acids
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chemistry* Drug Carriers Electrophoresis, Agar Gel Erythrocytes / drug effects Hemolysis Humans Hydrogen-Ion Concentration Methacrylates / chemistry*, pharmacology Nylons / chemistry*, pharmacology Particle Size Polyethylene Glycols / chemistry*, pharmacology Transfection* |
| Chemical | |
Reg. No./Substance:
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0/Acids; 0/Drug Carriers; 0/Methacrylates; 0/Nylons; 0/Polyethylene Glycols; 0/poly(2-(dimethylamino)ethyl methacrylate) |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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