| Inhibitor/fatty acid interactions with cytochrome P-450 BM3. | |
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MedLine Citation:
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PMID: 8914986 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The interaction of fatty acid substrate (palmitate) and inhibitor (metyrapone: 2-methyl-1,2-di-3-pyridyl-1-propanone) with cytochrome P-450 BM3 was analysed by UV-visible and circular dichroism spectroscopy, and by surface-enhanced resonance Raman scattering (SERRS). While visible spectroscopy provides information on the relative affinities of these compounds, SERRS provides additional novel data indicating palmitate-induced structural changes in the haem environment. SERRS also demonstrates that binding of both palmitate and the large nitrogenous ligand metyrapone occurs simultaneously to P-450 BM3 -- highlighting the usefulness of this technique in probing haemoprotein active sites. |
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Authors:
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I D Macdonald; W E Smith; A W Munro |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: FEBS letters Volume: 396 ISSN: 0014-5793 ISO Abbreviation: FEBS Lett. Publication Date: 1996 Nov |
Date Detail:
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Created Date: 1996-12-20 Completed Date: 1996-12-20 Revised Date: 2008-11-21 |
Medline Journal Info:
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Nlm Unique ID: 0155157 Medline TA: FEBS Lett Country: NETHERLANDS |
Other Details:
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Languages: eng Pagination: 196-200 Citation Subset: IM |
Affiliation:
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Department of Pure and Applied Chemistry, University of Strathclyde, Glasgow, UK. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Bacterial Proteins* Circular Dichroism Cytochrome P-450 Enzyme System / chemistry, metabolism* Enzyme Inhibitors / chemistry, metabolism Heme / chemistry, metabolism Metyrapone / chemistry, metabolism* Mixed Function Oxygenases / chemistry, metabolism* NADPH-Ferrihemoprotein Reductase Palmitic Acid / chemistry, metabolism* Spectrum Analysis Spectrum Analysis, Raman |
| Chemical | |
Reg. No./Substance:
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0/Bacterial Proteins; 0/Enzyme Inhibitors; 14875-96-8/Heme; 54-36-4/Metyrapone; 57-10-3/Palmitic Acid; 9035-51-2/Cytochrome P-450 Enzyme System; EC 1.-/Mixed Function Oxygenases; EC 1.6.2.4/NADPH-Ferrihemoprotein Reductase; EC 1.6.2.4/flavocytochrome P450 BM3 monoxygenases |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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