Document Detail


Is acetylcholine an autocrine/paracrine growth factor via the nicotinic alpha7-receptor subtype in the human colon cancer cell line HT-29?
MedLine Citation:
PMID:  19285065     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
We used immunochemistry to demonstrate expression of acetylcholine's nicotinic alpha7-receptor subtype in human colon cancer cell line HT-29. Moreover, RT-PCR and immunochemistry showed that choline acetyltransferase and acetylcholine esterase, the enzymes responsible for acetylcholine synthesis and degradation, respectively, localise in HT-29 cells. Bromoacetylcholine bromide, an inhibitor of choline acetyltransferase, significantly attenuated basal cell growth. Our findings suggest that acetylcholine might serve as an autocrine/paracrine-or speculatively, even intracrine-signalling molecule in cell line HT-29, thus contributing to carcinogenesis/cancer progression.
Authors:
Ann Pettersson; Linn Nilsson; Gunnar Nylund; Amir Khorram-Manesh; Svante Nordgren; Dick S Delbro
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2009-03-11
Journal Detail:
Title:  European journal of pharmacology     Volume:  609     ISSN:  1879-0712     ISO Abbreviation:  Eur. J. Pharmacol.     Publication Date:  2009 May 
Date Detail:
Created Date:  2009-04-21     Completed Date:  2009-07-13     Revised Date:  2010-06-04    
Medline Journal Info:
Nlm Unique ID:  1254354     Medline TA:  Eur J Pharmacol     Country:  Netherlands    
Other Details:
Languages:  eng     Pagination:  27-33     Citation Subset:  IM    
Affiliation:
School of Pure and Applied Natural Sciences, University of Kalmar, SE-39182 Kalmar, Sweden.
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MeSH Terms
Descriptor/Qualifier:
Acetylcholine / biosynthesis,  metabolism*
Acetylcholinesterase / metabolism
Autocrine Communication*
Biotinylation
Cell Line, Tumor
Choline O-Acetyltransferase / metabolism
Colonic Neoplasms / metabolism*
Fluorescent Antibody Technique, Indirect
HT29 Cells
Humans
Immunohistochemistry
Intercellular Signaling Peptides and Proteins / metabolism*
Paracrine Communication*
Receptors, Nicotinic / metabolism*
Chemical
Reg. No./Substance:
0/Intercellular Signaling Peptides and Proteins; 0/Receptors, Nicotinic; 0/alpha7 nicotinic acetylcholine receptor; 51-84-3/Acetylcholine; EC 2.3.1.6/Choline O-Acetyltransferase; EC 3.1.1.7/Acetylcholinesterase

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