Document Detail


An acetylation method for the quantification of membrane lipids, including phospholipids, polyphosphoinositides and cholesterol.
MedLine Citation:
PMID:  1848754     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
A method for the quantification of membrane lipids by acetylation with [3H]acetic anhydride is described. A standard lipid, labelled with 14C or 32P, is added to the sample and is simultaneously acetylated. The 3H/14C or 3H/32P ratios obtained in the acetylated lipids are proportional to the initial lipid concentration. The method has been used to quantify cholesterol, phosphatidylethanolamine, phosphatidylinositol, sphingomyelin, phosphatidylinositol 4-phosphate and phosphatidylinositol 4,5-bisphosphate in amounts between 2 and 10 nmol.
Authors:
J M Stein; G A Smith; J P Luzio
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Publication Detail:
Type:  Comparative Study; Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  The Biochemical journal     Volume:  274 ( Pt 2)     ISSN:  0264-6021     ISO Abbreviation:  Biochem. J.     Publication Date:  1991 Mar 
Date Detail:
Created Date:  1991-04-22     Completed Date:  1991-04-22     Revised Date:  2010-09-09    
Medline Journal Info:
Nlm Unique ID:  2984726R     Medline TA:  Biochem J     Country:  ENGLAND    
Other Details:
Languages:  eng     Pagination:  375-9     Citation Subset:  IM    
Affiliation:
Department of Clinical Biochemistry, University of Cambridge, Addenbrooke's Hospital, U.K.
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MeSH Terms
Descriptor/Qualifier:
Acetylation
Carbon Radioisotopes
Cell Fractionation
Cell Membrane / chemistry*,  ultrastructure
Centrifugation, Density Gradient
Cholesterol / analysis*,  blood
Chromatography, Thin Layer / methods
Colorimetry / methods
Humans
Indicators and Reagents
Membrane Lipids / analysis*,  blood
Neutrophils / chemistry*
Phosphatidylinositol Phosphates
Phosphatidylinositols / analysis*,  blood
Phospholipids / analysis*,  blood
Phosphorus Radioisotopes
Radioisotope Dilution Technique
Tritium
Chemical
Reg. No./Substance:
0/Carbon Radioisotopes; 0/Indicators and Reagents; 0/Membrane Lipids; 0/Phosphatidylinositol Phosphates; 0/Phosphatidylinositols; 0/Phospholipids; 0/Phosphorus Radioisotopes; 10028-17-8/Tritium; 57-88-5/Cholesterol
Comments/Corrections

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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