Document Detail


An accelerated method for isolation of Salmonella enterica serotype Typhimurium from artificially contaminated foods, using a short preenrichment, immunomagnetic separation, and xylose-lysine-desoxycholate agar (6IX method).
MedLine Citation:
PMID:  19343948     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Rapid isolation of Salmonella from food is essential for faster typing and source tracking in an outbreak. The objective of this study was to investigate a rapid isolation method that would augment the standard U.S. Food and Drug Administration's Bacteriological Analytical Manual (BAM) method. Food samples with low microbial load, including egg salad and ice cream, moderately high-microbial-load tomatoes, and high-microbial-load ground beef were intentionally inoculated with 2 to 48 CFU of Salmonella enterica serotype Typhimurium. The samples were preenriched in buffered peptone water for 6 h, and then selectively concentrated by immunomagnetic separation and plated for isolation on xylose-lysine-desoxycholate agar: the 6IX method. Salmonella Typhimurium was presumptively identified from approximately 97% of the low-microbial-load and moderately high-microbial-load samples by the 6IX method 2 days before the BAM standard method for isolation of Salmonella. In 49% of the beef samples, Salmonella Typhimurium was presumptively identified 1 or 2 days earlier by the 6IX method. Given the inocula used, our data clearly indicated that for most of the food samples tested, with the exception of ground beef, Salmonella Typhimurium could be isolated two laboratory days earlier with the 6IX method compared with the BAM method. In conclusion, this 6IX method may expedite Salmonella isolation and, therefore, has the potential to accelerate strain tracking for epidemiological analysis in a foodborne outbreak.
Authors:
Aparna Tatavarthy; Kealy Peak; William Veguilla; Teresa Cutting; Valerie J Harwood; Jill Roberts; Philip Amuso; Jacqueline Cattani; Andrew Cannons
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Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, Non-P.H.S.    
Journal Detail:
Title:  Journal of food protection     Volume:  72     ISSN:  0362-028X     ISO Abbreviation:  J. Food Prot.     Publication Date:  2009 Mar 
Date Detail:
Created Date:  2009-04-06     Completed Date:  2009-05-05     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  7703944     Medline TA:  J Food Prot     Country:  United States    
Other Details:
Languages:  eng     Pagination:  583-90     Citation Subset:  IM    
Affiliation:
Center for Biological Defense, College of Public Health, 3602 Spectrum Boulevard, University of South Florida, Tampa, Florida 33612, USA. atatavar@health.usf.edu
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MeSH Terms
Descriptor/Qualifier:
Agar / chemistry*
Colony Count, Microbial
Consumer Product Safety
Deoxycholic Acid / metabolism
Disease Outbreaks
Food Contamination / analysis*
Food Microbiology
Humans
Immunomagnetic Separation / methods*
Lysine / metabolism
Salmonella Food Poisoning / epidemiology,  prevention & control
Salmonella typhimurium / growth & development,  isolation & purification*,  metabolism
Sensitivity and Specificity
Species Specificity
Time Factors
Xylose / metabolism
Chemical
Reg. No./Substance:
0/Xylose; 56-87-1/Lysine; 83-44-3/Deoxycholic Acid; 9002-18-0/Agar

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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