| The absence of beta 2-microglobulin in Daudi cells: active gene but inactive messenger RNA. | |
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MedLine Citation:
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PMID: 6186604 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The Daudi cell line is characterized by an absence of HLA antigen on its surface. This has been attributed to a lack of beta 2-microglobulin (beta 2m) while the heavy chain of HLA is present intracellularly. Karyotype analysis of Daudi cells has shown a deletion involving one of the beta 2-microglobulin alleles. It was generally believed that the absence of beta 2-microglobulin in that cell line resulted from an absence of expression of the remaining gene. We report here the unexpected finding of a normal amount of beta 2-microglobulin messenger RNA in Daudi cells. This was demonstrated by "Northern blot" hybridization with cDNA plasmid clones as a probe. This mRNA, however, when purified by hybridization-selection with beta 2-microglobulin plasmid DNA, is unable to function as messenger in protein synthesis and is therefore an inactive mRNA. The finding of a translationally inactive beta 2-microglobulin mRNA provides a new explanation for the absence of beta 2-microglobulin and therefore of HLA antigens in Daudi cells. |
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Authors:
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C de Préval; B Mach |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Immunogenetics Volume: 17 ISSN: 0093-7711 ISO Abbreviation: Immunogenetics Publication Date: 1983 |
Date Detail:
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Created Date: 1983-04-21 Completed Date: 1983-04-21 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 0420404 Medline TA: Immunogenetics Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 133-40 Citation Subset: IM |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Beta-Globulins / genetics* Burkitt Lymphoma / genetics* Cell Line HLA Antigens / genetics Humans Mice RNA, Messenger / genetics, physiology* beta 2-Microglobulin / genetics* |
| Chemical | |
Reg. No./Substance:
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0/Beta-Globulins; 0/HLA Antigens; 0/RNA, Messenger; 0/beta 2-Microglobulin |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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