| The ability of walnut extract and fatty acids to protect against the deleterious effects of oxidative stress and inflammation in hippocampal cells. | |
| | |
MedLine Citation:
|
PMID: 23321679 Owner: NLM Status: Publisher |
Abstract/OtherAbstract:
|
Previous research from our lab has demonstrated that dietary walnut supplementation protects against age-related cognitive declines in rats; however, the cellular mechanisms by which walnuts and polyunsaturated fatty acids (PUFAs) may affect neuronal health and functioning in aging are undetermined. OBJECTIVES: We assessed if pretreatment of primary hippocampal neurons with walnut extract or PUFAs would protect cells against dopamine- and lipopolysaccharide-mediated cell death and calcium dysregulation. METHODS: Rat primary hippocampal neurons were pretreated with varying concentrations of walnut extract, linoleic acid, alpha-linolenic acid, eicosapentaenoic acid, or docosahexaenoic acid prior to exposure to either dopamine or lipopolysaccharide. Viability was assessed using the Live/Dead Cellular Viability/Cytotoxicity Kit. Also, the ability of the cells to return to baseline calcium levels after depolarization was measured with fluorescent imaging. RESULTS: Results indicated that walnut extract, alpha-linolenic acid, and docosahexaenoic acid provided significant protection against cell death and calcium dysregulation; the effects were pretreatment concentration dependent and stressor dependent. Linoleic acid and eicosapentaenoic acid were not as effective at protecting hippocampal cells from these insults. DISCUSSION: Walnut extract and omega-3 fatty acids may protect against age-related cellular dysfunction, but not all PUFAs are equivalent in their beneficial effects. |
| | |
Authors:
|
Amanda N Carey; Derek R Fisher; James A Joseph; Barbara Shukitt-Hale |
Related Documents
:
|
23626389 - Characterisation of polyphenols in terminalia arjuna bark extract. 9186559 - Pterulinic acid and pterulone, two novel inhibitors of nadh:ubiquinone oxidoreductase (... 15070349 - The dynamic structure of jadomycin b and the amino acid incorporation step of its biosy... 23007169 - The metabolic syndrome of fructose-fed rats: effects of long-chain polyunsaturated ω3 ... 23070949 - Effect of grafting and ripening conditions on some qualitative traits of 'cuore di bue... 23478969 - Effectiveness of disinfectants used in hemodialysis against both candida orthopsilosis ... 18960829 - Potentiometric determination of lead with dtpa in the presence of large amounts of alum... 11861929 - Efficacy of dietary arachidonic acid provided as triglyceride or phospholipid as substr... 17697859 - Efficacy and safety of allopurinol in patients with hypoxanthine-guanine phosphoribosyl... |
Publication Detail:
|
Type: JOURNAL ARTICLE Date: 2012-12-4 |
Journal Detail:
|
Title: Nutritional neuroscience Volume: - ISSN: 1476-8305 ISO Abbreviation: Nutr Neurosci Publication Date: 2012 Dec |
Date Detail:
|
Created Date: 2013-1-16 Completed Date: - Revised Date: - |
Medline Journal Info:
|
Nlm Unique ID: 100892202 Medline TA: Nutr Neurosci Country: - |
Other Details:
|
Languages: ENG Pagination: - Citation Subset: - |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
|
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: The effect of Hurricane Katrina on the prevalence of health impairments and disability among adults ...
Next Document: BRG1-mediated immune tolerance: facilitation of Treg activation and partial independence of chromati...