| The ability of adenosine to decrease the concentration of fructose 2,6-bisphosphate in isolated hepatocytes. A cyclic AMP-mediated effect. | |
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MedLine Citation:
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PMID: 6324747 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The presence of adenosine (25-250 microM) or of 2-chloroadenosine (2.5-100 microM) in the incubation medium caused a marked decrease in the concentration of fructose 2,6-bisphosphate in isolated hepatocytes. This effect was accompanied by an increase in the concentration of cyclic AMP, an activation of phosphorylase and of fructose 2,6-bisphosphatase, and an inactivation of pyruvate kinase and of 6-phosphofructo-2-kinase. As a rule, the changes in the fructose 2,6-bisphosphate-modifying system were slower but more persistent than those in the activities of phosphorylase and pyruvate kinase. The effect of the nucleoside to decrease the concentration of fructose 2,6-bisphosphate was not affected by an inhibitor of adenosine transport and could not be obtained in a liver high-speed supernatant. These data indicate that the effect of adenosine to decrease the concentration of fructose 2,6-bisphosphate is mediated by the stimulation of adenylate cyclase, secondary to the binding of adenosine to membranous receptors. Like glucagon, 2-chloroadenosine stimulated gluconeogenesis in isolated hepatocytes, whereas adenosine had an opposite effect. |
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Authors:
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R Bartrons; E Van Schaftingen; H G Hers |
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Publication Detail:
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Type: In Vitro; Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S. |
Journal Detail:
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Title: The Biochemical journal Volume: 218 ISSN: 0264-6021 ISO Abbreviation: Biochem. J. Publication Date: 1984 Feb |
Date Detail:
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Created Date: 1984-05-04 Completed Date: 1984-05-04 Revised Date: 2009-11-18 |
Medline Journal Info:
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Nlm Unique ID: 2984726R Medline TA: Biochem J Country: ENGLAND |
Other Details:
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Languages: eng Pagination: 157-63 Citation Subset: IM |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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2-Chloroadenosine Adenosine / analogs & derivatives, pharmacology* Adenylate Cyclase / metabolism Animals Cyclic AMP / metabolism Depression, Chemical Dose-Response Relationship, Drug Fructosediphosphates / metabolism* Glucagon / pharmacology Gluconeogenesis / drug effects Hexosediphosphates / metabolism* Liver / drug effects, metabolism* Male Rats Rats, Inbred Strains |
| Grant Support | |
ID/Acronym/Agency:
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AM 9235/AM/NIADDK NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/Fructosediphosphates; 0/Hexosediphosphates; 146-77-0/2-Chloroadenosine; 58-61-7/Adenosine; 60-92-4/Cyclic AMP; 79082-92-1/fructose 2,6-diphosphate; 9007-92-5/Glucagon; EC 4.6.1.1/Adenylate Cyclase |
| Comments/Corrections | |
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