Document Detail

The abc1 ( - )/coq8 ( - ) respiratory-deficient mutant of Schizosaccharomyces pombe suffers from glutathione underproduction and hyperaccumulates Cd (2+).
MedLine Citation:
PMID:  21818608     Owner:  NLM     Status:  Publisher    
The abc1 ( - )/coq8 ( - ) gene deletion respiratory-deficient mutant NBp17 of fission yeast Schizosaccharomyces pombe displayed a phenotypic fermentation pattern with enhanced production of glycerol and acetate, and also possessed oxidative stress-sensitive phenotypes to H(2)O(2), menadione, tBuOOH, Cd(2+), and chromate in comparison with its parental respiratory-competent strain HNT. As a consequence of internal stress-inducing mutation, adaptation processes to restore the redox homeostasis of mutant NBp17 cells were detected in minimal glucose medium. Mutant NBp17 produced significantly increased amounts of O (2) (•-) and H(2)O(2) as a result of the decreased internal glutathione concentration and the only slightly increased glutathione reductase activity. The Cr(VI) reduction capacity and hence the (•)OH production ability were decreased. The mutant cells demonstrated increased specific activities of superoxide dismutases and glutathione reductase (but not catalase) to detoxify at least partially the overproduction of reactive oxygen species. All these features may be explained by the decreased redox capacity of the mutant cells. Most notably, mutant NBp17 hyperaccumulated yellow CdS.
Zoltan Gazdag; Stefan Fujs; Balázs Kőszegi; Nikoletta Kálmán; Gábor Papp; Tamás Emri; Joseph Belágyi; István Pócsi; Peter Raspor; Miklós Pesti
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Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2011-8-5
Journal Detail:
Title:  Folia microbiologica     Volume:  -     ISSN:  1874-9356     ISO Abbreviation:  -     Publication Date:  2011 Aug 
Date Detail:
Created Date:  2011-8-5     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0376757     Medline TA:  Folia Microbiol (Praha)     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Department of General and Environmental Microbiology, Faculty of Sciences, University of Pécs, 7602, Pécs, P.O. Box 266, Hungary.
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