Document Detail


Zygotic development without functional mitotic centrosomes.
MedLine Citation:
PMID:  11231128     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The centrosome is the dominant microtubule-organizing center in animal cells. At the onset of mitosis, each cell normally has two centrosomes that lie on opposite sides of the nucleus. Centrosomes nucleate the growth of microtubules and orchestrate the efficient assembly of the mitotic spindle. Recent studies in vivo and in vitro have shown that the spindle can form even in the absence of centrosomes and demonstrate that individual cells can divide without this organelle. However, since centrosomes are involved in multiple processes in vivo, including polarized cell divisions, which are an essential developmental mechanism for producing differentiated cell types, it remains to be shown whether or not a complete organism can develop without centrosomes. Here we show that in Drosophila a centrosomin (cnn) null mutant, which fails to assemble fully functional mitotic centrosomes and has few or no detectable astral microtubules, can develop into an adult fly. These results challenge long-held assumptions that the centrosome and the astral microtubules emanating from it are essential for development and are required specifically for spindle orientation during asymmetric cell divisions.
Authors:
T L Megraw; L R Kao; T C Kaufman
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, Non-P.H.S.    
Journal Detail:
Title:  Current biology : CB     Volume:  11     ISSN:  0960-9822     ISO Abbreviation:  Curr. Biol.     Publication Date:  2001 Jan 
Date Detail:
Created Date:  2001-03-20     Completed Date:  2001-06-07     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  9107782     Medline TA:  Curr Biol     Country:  England    
Other Details:
Languages:  eng     Pagination:  116-20     Citation Subset:  IM    
Affiliation:
Department of Biology and Howard Hughes Medical Institute, Indiana University, Bloomington, IN 47405, USA. tmegraw@bio.indiana.edu
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MeSH Terms
Descriptor/Qualifier:
Animals
Centrosome*
Drosophila / cytology,  embryology*,  genetics
Mitosis*
Zygote / growth & development*

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