| Zyg-11 and cul-2 regulate progression through meiosis II and polarity establishment in C. elegans. | |
| | |
MedLine Citation:
|
PMID: 15215208 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
The mechanisms that ensure coupling between meiotic cell cycle progression and subsequent developmental events, including specification of embryonic axes, are poorly understood. Here, we establish that zyg-11 and the cullin cul-2 promote the metaphase-to-anaphase transition and M phase exit at meiosis II in Caenorhabditis elegans. Our results indicate that ZYG-11 acts with a CUL-2-based E3 ligase that is essential at meiosis II and that functions redundantly with the anaphase-promoting complex/cyclosome at meiosis I. Our data also indicate that delayed M phase exit in zyg-11(RNAi) embryos is due to accumulation of the B type cyclin CYB-3. We demonstrate that PAR proteins and P granules become polarized in an inverted manner during the meiosis II delay resulting from zyg-11 or cul-2 inactivation, and that zyg-11 and cul-2 can regulate polarity establishment independently of a role in cell cycle progression. Furthermore, we find that microtubules appear dispensable for ectopic polarity during the meiosis II delay in zyg-11(RNAi) embryos, as well as for AP polarity during the first mitotic cell cycle in wild-type embryos. Our findings suggest a model in which a CUL-2-based E3 ligase promotes cell cycle progression and prevents polarity establishment during meiosis II, and in which the centrosome acts as a cue to polarize the embryo along the AP axis after exit from the meiotic cell cycle. |
| | |
Authors:
|
Rémi Sonneville; Pierre Gönczy |
Related Documents
:
|
17340618 - Antiproliferative activity of ccn3: involvement of the c-terminal module and post-trans... 16418578 - Gw bodies, micrornas and the cell cycle. 18205808 - Oxidant-induced cell-cycle delay in saccharomyces cerevisiae: the involvement of the sw... 20571068 - The dynamin inhibitors mitmab and octmab induce cytokinesis failure and inhibit cell pr... 15292258 - Heparan sulfate/heparin oligosaccharides protect stromal cell-derived factor-1 (sdf-1)/... 12706498 - Aldh3a1 protects human corneal epithelial cells from ultraviolet- and 4-hydroxy-2-nonen... |
Publication Detail:
|
Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2004-06-23 |
Journal Detail:
|
Title: Development (Cambridge, England) Volume: 131 ISSN: 0950-1991 ISO Abbreviation: Development Publication Date: 2004 Aug |
Date Detail:
|
Created Date: 2004-07-20 Completed Date: 2004-09-30 Revised Date: 2009-11-19 |
Medline Journal Info:
|
Nlm Unique ID: 8701744 Medline TA: Development Country: England |
Other Details:
|
Languages: eng Pagination: 3527-43 Citation Subset: IM |
Affiliation:
|
ISREC (Swiss Institute for Experimental Cancer Research Boveresses, CH-1066 Epalinges/Lausanne, Switzerland. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Animals Biological Markers Body Patterning* Caenorhabditis elegans / embryology* Caenorhabditis elegans Proteins / genetics, metabolism* Cell Cycle Proteins / genetics, metabolism* Cell Polarity Cullin Proteins / genetics, metabolism* Cyclin B / genetics, metabolism* Cyclin B1 Cytoskeleton / metabolism Female Meiosis / physiology* Microtubules / metabolism Morphogenesis Nuclear Proteins / metabolism RNA Interference Recombinant Fusion Proteins / genetics, metabolism |
| Chemical | |
Reg. No./Substance:
|
0/Biological Markers; 0/CYB-3 protein, C elegans; 0/Caenorhabditis elegans Proteins; 0/Cell Cycle Proteins; 0/Cullin Proteins; 0/Cyclin B; 0/Cyclin B1; 0/Nuclear Proteins; 0/Recombinant Fusion Proteins; 0/Zyg11 protein, C elegans; 0/cullin-2 protein, C elegans |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Transduction of graded Hedgehog signaling by a combination of Gli2 and Gli3 activator functions in t...
Next Document: CUL-2 and ZYG-11 promote meiotic anaphase II and the proper placement of the anterior-posterior axis...