| Zwittermicin A resistance gene from Bacillus cereus. | |
| | |
MedLine Citation:
|
PMID: 8763956 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
Zwittermicin A is a novel aminopolyol antibiotic produced by Bacillus cereus that is active against diverse bacteria and lower eukaryotes (L.A. Silo-Suh, B.J. Lethbridge, S.J. Raffel, H. He, J. Clardy, and J. Handelsman, Appl. Environ. Microbiol. 60:2023-2030, 1994). To identify a determinant for resistance to zwittermicin A, we constructed a genomic library from B. cereus UW85, which produces zwittermicin A, and screened transformants of Escherichia coli DH5alpha, which is sensitive to zwittermicin A, for resistance to zwittermicin A. Subcloning and mutagenesis defined a genetic locus, designated zmaR, on a 1.2-kb fragment of DNA that conferred zwittermicin A resistance on E. coli. A DNA fragment containing zmaR hybridized to a corresponding fragment of genomic DNA from B. cereus UW85. Corresponding fragments were not detected in mutants of B. cereus UW85 that were sensitive to zwittermicin A, and the plasmids carrying zmaR restored resistance to the zwittermicin A-sensitive mutants, indicating that zmaR was deleted in the zwittermicin A-sensitive mutants and that zmaR is functional in B. cereus. Sequencing of the 1.2-kb fragment of DNA defined an open reading frame, designated ZmaR. Neither the nucleotide sequence nor the predicted protein sequence had significant similarity to sequences in existing databases. Cell extracts from an E. coli strain carrying zmaR contained a 43.5-kDa protein whose molecular mass and N-terminal sequence matched those of the protein predicted by the zmaR sequence. The results demonstrate that we have isolated a gene, zmaR, that encodes a zwIttermicin A resistance determinant that is functional in both B. cereus and E. coli. |
| | |
Authors:
|
J L Milner; E A Stohl; J Handelsman |
Related Documents
:
|
10594226 - Overexpression of the cat-86 gene is associated with thermosensitivity in bacillus subt... 2181026 - Resistance to bacillus thuringiensis by the indian meal moth, plodia interpunctella: co... 1499986 - Electrochemical sterilization of bacteria absorbed on granular activated carbon. 3098166 - Toxicity of paraquat to microorganisms. 21292906 - Emergence of community-associated methicillin-resistant staphylococcus aureus carriage ... 2223606 - Mycoplasma lactucae sp. nov., a sterol-requiring mollicute from a plant surface. |
Publication Detail:
|
Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
|
Title: Journal of bacteriology Volume: 178 ISSN: 0021-9193 ISO Abbreviation: J. Bacteriol. Publication Date: 1996 Jul |
Date Detail:
|
Created Date: 1996-10-01 Completed Date: 1996-10-01 Revised Date: 2010-09-10 |
Medline Journal Info:
|
Nlm Unique ID: 2985120R Medline TA: J Bacteriol Country: UNITED STATES |
Other Details:
|
Languages: eng Pagination: 4266-72 Citation Subset: IM |
Affiliation:
|
Department of Plant Pathology, University of Wisconsin-Madison 53706, USA. |
| Data Bank Information | |
Bank Name/Acc. No.:
|
GENBANK/U57065 |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Acetyltransferases* Amino Acid Sequence Anti-Bacterial Agents / pharmacology* Bacillus cereus / drug effects, genetics* Bacterial Proteins / genetics* Base Sequence Cosmids Drug Resistance, Microbial / genetics Gene Library Genes, Bacterial* Molecular Sequence Data Peptides* Phenotype Sequence Analysis, DNA Sequence Deletion |
| Chemical | |
Reg. No./Substance:
|
0/Anti-Bacterial Agents; 0/Bacterial Proteins; 0/Peptides; 155547-95-8/zwittermicin A; EC 2.3.1.-/Acetyltransferases; EC 2.3.1.-/ZmaR protein, Bacillus |
| Comments/Corrections | |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Replication fork arrest at relocated replication terminators on the Bacillus subtilis chromosome.
Next Document: Identification of the promoter region for the colanic acid polysaccharide biosynthetic genes in Esch...