| Zonation of hepatic bile salt transporters. | |
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MedLine Citation:
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PMID: 16614971 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Pericentral and periportal hepatocytes differ in their capacity to eliminate and velocity of eliminating bile acids and other organic anions. We wonder whether differences in the distribution of anion transporters (ntcp [M77479], besp [NM_031760], mrp2 [NM_012833], oatp1 [NM_017111], oatp2 [NM_131906]) cause the differences in bile acid excretion. Therefore, we analyzed the distribution of these anion transporters in periportal and pericentral cells by immunohistology, their mRNA by quantitative PCR, and regulating nuclear factors (NF-kappaB, HNF1, HNF3, HNF4, FXR, PXR) by gel shift assay. We did not find any differences in nuclear factors or regarding the proteins that could explain the zonal differences in anion transport. |
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Authors:
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P K Baier; S Hempel; B Waldvogel; U Baumgartner |
Publication Detail:
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Type: Comparative Study; Journal Article |
Journal Detail:
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Title: Digestive diseases and sciences Volume: 51 ISSN: 0163-2116 ISO Abbreviation: Dig. Dis. Sci. Publication Date: 2006 Mar |
Date Detail:
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Created Date: 2006-04-14 Completed Date: 2006-05-17 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 7902782 Medline TA: Dig Dis Sci Country: United States |
Other Details:
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Languages: eng Pagination: 587-93 Citation Subset: AIM; IM |
Affiliation:
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Department of Surgery, University of Freiburg, Freiburg, Germany. baier@chir.ukl.uni-freiburg.de |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Bile Acids and Salts / analysis, metabolism* Biological Transport / physiology Cells, Cultured / cytology Female Glutathione Synthase / metabolism Hepatocyte Nuclear Factors / analysis, metabolism* Hepatocytes / metabolism* Immunohistochemistry Male Models, Animal Organic Anion Transporters / analysis, metabolism* Polymerase Chain Reaction / methods RNA, Messenger / analysis Rats Rats, Sprague-Dawley Sensitivity and Specificity |
| Chemical | |
Reg. No./Substance:
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0/Bile Acids and Salts; 0/Hepatocyte Nuclear Factors; 0/Organic Anion Transporters; 0/RNA, Messenger; EC 6.3.2.3/Glutathione Synthase |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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