Document Detail

Zonation of hepatic bile salt transporters.
MedLine Citation:
PMID:  16614971     Owner:  NLM     Status:  MEDLINE    
Pericentral and periportal hepatocytes differ in their capacity to eliminate and velocity of eliminating bile acids and other organic anions. We wonder whether differences in the distribution of anion transporters (ntcp [M77479], besp [NM_031760], mrp2 [NM_012833], oatp1 [NM_017111], oatp2 [NM_131906]) cause the differences in bile acid excretion. Therefore, we analyzed the distribution of these anion transporters in periportal and pericentral cells by immunohistology, their mRNA by quantitative PCR, and regulating nuclear factors (NF-kappaB, HNF1, HNF3, HNF4, FXR, PXR) by gel shift assay. We did not find any differences in nuclear factors or regarding the proteins that could explain the zonal differences in anion transport.
P K Baier; S Hempel; B Waldvogel; U Baumgartner
Publication Detail:
Type:  Comparative Study; Journal Article    
Journal Detail:
Title:  Digestive diseases and sciences     Volume:  51     ISSN:  0163-2116     ISO Abbreviation:  Dig. Dis. Sci.     Publication Date:  2006 Mar 
Date Detail:
Created Date:  2006-04-14     Completed Date:  2006-05-17     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  7902782     Medline TA:  Dig Dis Sci     Country:  United States    
Other Details:
Languages:  eng     Pagination:  587-93     Citation Subset:  AIM; IM    
Department of Surgery, University of Freiburg, Freiburg, Germany.
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MeSH Terms
Bile Acids and Salts / analysis,  metabolism*
Biological Transport / physiology
Cells, Cultured / cytology
Glutathione Synthase / metabolism
Hepatocyte Nuclear Factors / analysis,  metabolism*
Hepatocytes / metabolism*
Models, Animal
Organic Anion Transporters / analysis,  metabolism*
Polymerase Chain Reaction / methods
RNA, Messenger / analysis
Rats, Sprague-Dawley
Sensitivity and Specificity
Reg. No./Substance:
0/Bile Acids and Salts; 0/Hepatocyte Nuclear Factors; 0/Organic Anion Transporters; 0/RNA, Messenger; EC Synthase

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