| Zona pellucida as physiological trigger for the induction of acrosome reaction. | |
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MedLine Citation:
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PMID: 9739426 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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To evaluate the kinetics of acrosome reaction, sperm samples from four fertile donors were prepared by swim-up and incubated with solutions of human zonae containing 0.1, 0.15, 0.3, 0.5 and 1.0 zonae microliter-1. After 20, 40 and 60 min of incubation at 37 degrees C, aliquots were taken for evaluation of the acrosomal status. The results showed a distinct time- and dose dependence of the acrosome reaction induced by solubilized zona proteins. After 60 min of incubation in 1.0 zonae microliter-1, about 80% of the spermatozoa showed signs of acrosomal loss; about 40% were completely acrosome-reacted. In addition, zona-bound sperm showed the same ratios of acrosome-reacted spermatozoa in control experiments. The velocity of acrosome reaction was calculated by means of a double-reciprocal plot being 2.0-2.5% min-1 for completely reacted spermatozoa and those showing signs of acrosome reaction. However, both subgroups differed considerably in their constants of equilibrium (K = 2.0 ZP microliter-1 and K = 0.2 ZP microliter-1, respectively). In nonreacted and partly reacted spermatozoa results might indicate a disturbed course of acrosome reaction or possibly the existence of different subpopulations in respect of sperm competition. |
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Authors:
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R Henkel; D R Franken; U F Habenicht |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Andrologia Volume: 30 ISSN: 0303-4569 ISO Abbreviation: Andrologia Publication Date: 1998 Aug-Sep |
Date Detail:
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Created Date: 1999-01-19 Completed Date: 1999-01-19 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 0423506 Medline TA: Andrologia Country: GERMANY |
Other Details:
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Languages: eng Pagination: 275-80 Citation Subset: IM |
Affiliation:
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Center of Dermatology and Andrology, Justus Liebig University, Giessen, Germany. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Acrosome Reaction
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physiology* Female Humans Male Zona Pellucida / physiology* |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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