Document Detail

Zona pellucida as physiological trigger for the induction of acrosome reaction.
MedLine Citation:
PMID:  9739426     Owner:  NLM     Status:  MEDLINE    
To evaluate the kinetics of acrosome reaction, sperm samples from four fertile donors were prepared by swim-up and incubated with solutions of human zonae containing 0.1, 0.15, 0.3, 0.5 and 1.0 zonae microliter-1. After 20, 40 and 60 min of incubation at 37 degrees C, aliquots were taken for evaluation of the acrosomal status. The results showed a distinct time- and dose dependence of the acrosome reaction induced by solubilized zona proteins. After 60 min of incubation in 1.0 zonae microliter-1, about 80% of the spermatozoa showed signs of acrosomal loss; about 40% were completely acrosome-reacted. In addition, zona-bound sperm showed the same ratios of acrosome-reacted spermatozoa in control experiments. The velocity of acrosome reaction was calculated by means of a double-reciprocal plot being 2.0-2.5% min-1 for completely reacted spermatozoa and those showing signs of acrosome reaction. However, both subgroups differed considerably in their constants of equilibrium (K = 2.0 ZP microliter-1 and K = 0.2 ZP microliter-1, respectively). In nonreacted and partly reacted spermatozoa results might indicate a disturbed course of acrosome reaction or possibly the existence of different subpopulations in respect of sperm competition.
R Henkel; D R Franken; U F Habenicht
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Andrologia     Volume:  30     ISSN:  0303-4569     ISO Abbreviation:  Andrologia     Publication Date:    1998 Aug-Sep
Date Detail:
Created Date:  1999-01-19     Completed Date:  1999-01-19     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0423506     Medline TA:  Andrologia     Country:  GERMANY    
Other Details:
Languages:  eng     Pagination:  275-80     Citation Subset:  IM    
Center of Dermatology and Andrology, Justus Liebig University, Giessen, Germany.
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MeSH Terms
Acrosome Reaction / physiology*
Zona Pellucida / physiology*

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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